Tensor 27 ir fourier spectrometer

A Tensor 27 IR Fourier spectrometer (Bruker, Ettlingen, Germany) with an MCT detector was used to obtain the FTIR spectra of TsA and its conjugates. The measurements were carried out with a BioATR II thermostated cell using a single-reflection ZnSe element at 22 °C and FTIR microscope MICRAN-3. An aliquot (40 µL) of the corresponding enzyme solution (1.0–1.5 mg/mL in 10 mM sodium phosphate buffer) was applied to the internal reflection element, and the spectrum was recorded three times in the range from 3000 to 950 cm⁻¹ with a resolution of 1 cm⁻¹; we performed 50-fold scanning and averaging. The background was registered in the same way and automatically subtracted by the program. The resulting spectra were smoothed by the Savitzky–Golay method to a spectral resolution of 2 cm⁻¹ [33 (link)]. The spectra were analyzed using Opus 7.0 software (Bruker, Ettlingen, Germany).

The FTIR spectra of RrA and its conjugates were recorded using a Tensor 27 IR Fourier spectrometer (Bruker, Ettlingen, Germany) equipped with an MCT detector cooled with liquid nitrogen and a thermostat (Huber, Offenburg, Germany). The measurements were carried out in a BioATR II thermostated cell (Bruker, Ettlingen, Germany) using a single reflection ZnSe element at 22 °C and continuous purging of the system with dry air using a compressor JUN-AIR (Gast Inc., Benton Harbor, MI, USA). An aliquot (40 µL) of the corresponding enzyme solution (0.5–1.5 mg/mL in 10 mM sodium phosphate buffer) was applied to the internal reflection element, the spectrum was recorded three times in the range from 4000 to 950 cm⁻¹ with a resolution of 1 cm⁻¹; performed 70-fold scanning and averaging. The background was registered in the same way and was automatically subtracted by the program. The resulting spectra were smoothed by the Savitzky–Golay method [40 (link)] to a spectral resolution of 2 cm⁻¹. The spectra were analyzed using the Opus 7.0 software (Bruker, Ettlingen, Germany).

IR spectra were obtained on the Tensor 27 IR-Fourier spectrometer (Bruker, Germany) in the transmission mode using the KBr pressing method in the range of 350–4000 cm⁻¹ with a resolution of 4 cm⁻¹.

FTIR spectra of PLA/PCL blend films were recorded with a NETZSCH TG 209 F1 Libra thermoanalytical balance (NETZSCH-Gerätebau GmbH, Selb, Germany) and a Bruker Tensor 27 IR Fourier spectrometer (Bruker Corporation, Billerica, MA, USA), combined with a PIKE MIRacle™ accessory (PIKE Technologies, Madison, WI, USA). This attachment is equipped with a Teflon cell with Ge crystal and ATR plates, which allows the measurements of solid samples. The samples were tightly clamped to the surface of the Ge crystal to ensure optical contact. IR spectra were recorded in the range of 4000–400 cm⁻¹ with a resolution of 4 cm⁻¹ and averaged over 16 consecutive scans.

The IR spectra were recorded using a Tensor 27 spectrometer (Bruker) with an ATR (Pike MIRacle) unit on a germanium crystal (resolution was 4 cm⁻¹).
Spectra were recorded using a Bruker Tensor 27 IR Fourier spectrometer with an ATR attachment with a fixed angle of reflection (Pike MIRacle). The resolution was $4{\text{cm}}^{-1}$ .