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R zinc 3573

Manufactured by Merck Group
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Sourced in United States

(R)‐ZINC‐3573 is a laboratory reagent provided by Merck Group. It is a chemical compound with a specific molecular structure and properties. The core function of this product is to serve as a tool for researchers and scientists in various laboratory applications. No further details or interpretations about its intended use are provided.

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Lab products found in correlation

Meglumine diatrizoate, by Merck Group (2 mentions) Gadobutrol, by Merck Group (2 mentions) Evans blue, by Tecan (1 mentions) Evans blue, by Merck Group (1 mentions) Ketamine, by Ceva (1 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Mouse stem cell factor, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Levofloxacin, by Merck Group (1 mentions) Compound 48 80, by Merck Group (1 mentions)

3 protocols using r zinc 3573

1

Evaluating IgE-Mediated Vascular Permeability

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Mice were anesthetized through an i.p. injection of Ketamine (80 mg/kg; Ceva, USA). Human IgE stimulation was performed by s.c. injecting human IgE mAb (60 ng/mouse; Calbiochem, USA) at the base of the ear. Twenty‐four hours after sensitization, mice were injected IV with 50 µL of 12.5 mg/mL Evans blue (Sigma–Aldrich). After 10 min, mice were i.p. injected with anti‐human IgE (50 µg in 100 µL/mouse; Dako, USA). After 15 min, dorsal skin and ears were collected. To stimulate MRGPRX2 receptor after Evans blue injection, 10 µL of a solution containing 1 µM of meglumine diatrizoate (Sigma–Aldrich), gadobutrol (Sigma–Aldrich), and (R)‐ZINC‐3573 (Sigma–Aldrich) were administered by intraplantar injection in one paw and the vehicle was administered in the other paw. Evans blue extravasation was visualized 15–20 min later and paws were collected. Samples were dried for 24 h at 55–60°C, weighed, Evans blue was extracted by a 24‐h incubation in formamide at 55–60°C and quantified by the absorbance at OD620nm (Tecan, USA).
Mencarelli A., Gunawan M., Yong K.S., Bist P., Tan W.W., Tan S.Y., Liu M., Huang E.K., Fan Y., Chan J.K., Choi H.W., Abraham S.N, & Chen Q. (2020). A humanized mouse model to study mast cells mediated cutaneous adverse drug reactions. Journal of Leukocyte Biology, 107(5), 797-807.
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2

Preparation and Use of Lactic Acid/Lactate Solutions

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Dulbecco’s Modified Eagle’s Media (DMEM), Iscove’s Modified Dulbecco’s Media (IMDM), penicillin, streptomycin and L-glutamine supplement were purchased from Corning Cellgro™ (Corning, NY, United States). Recombinant human stem cell factor (hSCF) and mouse stem cell factor (mSCF) was purchased from PeproTech (Rocky Hill, NJ, United States). Opti-MEM™, Stem-ProTM-34 SFM media, and TRIzol™ were purchased from Invitrogen (Carlsbad, CA, United States). Chemical reagents used in buffers, unless otherwise noted, were purchased from Sigma-Aldrich (St. Louis, MO, United States). Compound 48/80, substance P (Arg-Pro-Lys-Pro-D-Phe-Gln-D-Trp-Phe-D-Trp-Leu-Leu) and (R)-ZINC-3573 were obtained from Sigma-Aldrich. Cathelicidin LL-37 (Leu-Leu-Gly-Asp-Phe-Phe-Arg-Lys-SerLys-Glu-Lys-Ile-Gly-Lys-Glu-Phe-Lys-Arg-Ile-Val-Gln-ArgIle-Lys-Asp-Phe-Leu-Arg-Asn-Leu-Val-Pro-Arg-Thr-Glu-Ser) was purchased from Tocris Bioscience (Minneapolis, MN, United States). Lactic acid and sodium lactate were purchased from Sigma-Aldrich as certified reference materials. Dilutions of lactic acid and sodium lactate were prepared in media, Siraganian (SIR) Buffer (118 mM NaCl, 5 mM KCl, 25 mM HEPES, 5.5 mM glucose, 0.4 mM MgCl2, 1 mM CaCl2, 0.1% w/v bovine serum albumin), or phosphate buffered saline (PBS).
Syed M., Kammala A.K., Callahan B., Oskeritzian C.A, & Subramanian H. (2021). Lactic acid suppresses MRGPRX2 mediated mast cell responses. Cellular immunology, 368, 104422.
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3

Immunoassay Reagents and Compounds

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Compound 48/80 (Sigma–Aldrich), levofloxacin (Sigma–Aldrich), meglumine diatrizoate (Sigma–Aldrich), gadobutrol (Sigma–Aldrich), (R)‐ZINC‐3573 (Sigma–Aldrich), human IgE (CalbioChem, USA), and rabbit polyclonal anti‐human IgE (Dako, USA) were obtained from respective companies.
Mencarelli A., Gunawan M., Yong K.S., Bist P., Tan W.W., Tan S.Y., Liu M., Huang E.K., Fan Y., Chan J.K., Choi H.W., Abraham S.N, & Chen Q. (2020). A humanized mouse model to study mast cells mediated cutaneous adverse drug reactions. Journal of Leukocyte Biology, 107(5), 797-807.
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