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5 protocols using phenylbis 2 4 6 trimethyl benzoyl phosphine oxide irgacure 819

1

Alumina Powder Synthesis and Functionalization

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In this experiment, three alumina powders with different average particle sizes were used: 100 nm (26N-0811UPA, Inframat Advanced Materials, Manchester, CT, USA), 500 nm (AES-11H, Sumitomo, Tokyo, Japan), and 2 μm (AES-23, Sumitomo). Reported properties of alumina powders are summarized in Table 1. Methyl alcohol (CH3OH, Extra pure, DEAJUNG, Siheung-si, Korea) was used as a solvent. The silane coupling agent was 3-trimethoxy-silylpropane-1-thiol (MPTMS), with an average molecular weight of Mw = 196.34 g/mol (Product No. KBM 503, Shin-Etsu Chemicals Co., Ltd., Tokyo, Japan). The monomer was trimethylolpropane triacrylate (TMPTA), with an average molecular weight of Mw = 296.32 g/mol. As a PI, phenylbis (2,4,6-trimethyl benzoyl) phosphine oxide (Irgacure 819) was purchased from Sigma Aldrich.
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2

Synthesis and Characterization of Photopolymer Composite

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1,6-Hexanediol diacrylate (HDDA), phenylbis(2,4,6-trimethylbenzoyl) phosphine oxide (Irgacure 819), 1-phenylazo-2-naphthol (Sudan I), and 3-(trimethoxysilyl) propyl methacrylate (TMSPM) were purchased from Sigma Aldrich. NaOH, methanol, and isopropanol were obtained from Merck.
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3

Fabrication of Piezoelectric Nanocomposites

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Chemicals utilized to formulate the resins are all commercially available and used as received. Barium titanate(IV) (nanopowder (cubic), 50 nm (SEM), 99.9% trace metals basis), barium titanate(IV) (nanopowder, cubic crystalline phase, <100 nm particle size, ≥99% trace metals basis), poly(ethylene glycol) diacrylate (PEGDA, average Mn 700), 3-(trimethoxysilyl)propyl methacrylate (TMSPMA, 98%), acetic acid (glacial, ACS reagent, ≥99.7%), phenylbis(2,4,6-trimethylbenzoyl)phosphine oxide (Irgacure 819, 97%), and 2-(2H-benzotriazol-2-yl)-6-dodecyl-4-methylphenol (Tinuvin 171) were purchased from Sigma-Aldrich. Ethanol (99.5%, anhydrous, 200 proof, ACROS Organics), 2-propanol (IPA, Certified ACS, Fisher Chemical), and Sylgard 184 silicone elastomer kit were purchased from Fisher Scientific. Lead zirconate titanate (PZT, nanopowder, purity: >99.5%, size: <100 nm) was purchased from Nanografi Nano Technology. Aluminum nitride (AlN, nanopowder, hexagonal, <40 nm) was purchased from Skyspring Nanomaterials. Conductive silver paste was purchased from Ted Pella.
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4

Fabrication of Hydrophobic Microparticles

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Poly (ethylene glycol) diacrylate (PEGDA, MW 250), phenylbis (2,4,6-trimethylbenzoyl) phosphine oxide (Irgacure 819), fluorescein and hydrogen fluoride (HF) were purchased from Sigma–Aldrich (St. Louis, MO, USA). 2-nitrophenyl phenyl sulfide (NPS) was purchased from TCI (Tokyo, Japan). Envy green polystyrene beads was purchased from Bangs Laboratories (Fishers, IN, USA). Isopropanol (IPA) was purchased from Fisher Scientific (Pittsburgh, PA, USA). Ultra-Ever Dry super-hydrophobic coating was purchased from Ultratech. Water was purified using a Millipore purification system (Bedford, MA, USA).
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5

Hydrogel Biomaterial Fabrication

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Polyethylene glycol diacrylate (PEGDA, 258 Da MW), phenylbis (2,4,6-trimethylbenzoyl)-phosphine oxide (Irgacure 819), 3-(trimethoxysilyl)propyl methacrylate, hydroxypropyl cellulose (HPC, 300 kDa), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Milwaukee, WI). 2-Nitrodiphenylsulfide (NPS) was from TCI America (Portland, OR). AlexaFluor 532 NHS ester (AF), acetone, and 2-propanol (IPA) were purchased from Fisher Scientific (Salt Lake City, UT). Glycine (G), ferritin (Fer), hydrochloric acid, sodium carbonate, and Amicon ultra 0.5 mL centrifuge filters were from Millipore Sigma (Billerica, MA). Sodium hydroxide was acquired from Mallinckrodt Specialty Chemicals (St. Louis, MO). Sodium bicarbonate monobasic was purchased from Merck (Burlington, MA). Toluene and glass microscope slides (3 × 1 in, 1 mm thick) were from Avantor (Center Valley, PA). Phenylalanine (F) was obtained from Spectrum Chemical (New Brunswick, NJ). PTB Peptide 1 (P1) and PTB Peptide 2 (P2) [39 (link)] were acquired from Biomatik (Wilmington, DE). Corticotropin releasing factor (CRF) was purchased from GeneScript (Piscataway, NJ). Lactoferrin (LF) was from Sigma (St. Louis, MO). Water purified to 18.3 MΩ·cm by a Barnstead EASYpure UV/UF system (Dubuque, IA) was used to prepare all solutions.
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