Diazepam

Manufactured by Lipomed

Sourced in Switzerland

Diazepam is a benzodiazepine compound used as a reference standard in analytical laboratories. It is a crystalline solid used for the identification and quantification of diazepam in various matrices through analytical techniques such as chromatography and spectroscopy.

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Diazepam-d5 (4 citations)

4 protocols using diazepam

Simultaneous LCMS Analysis of Psychoactive Drugs

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Standard solutions of the analytes alprazolam (ALP), citalopram (CIT), diazepam (DIAZ), doxepin (DOX), flunitrazepam (FLUN), fluoxetine (FLUOX), carbamazepine (CARB), cocaethylene (COCET), cocaine (COC), norcocaine (NORCOC), nordiazepam (NORDIAZ), nortriptyline (NORTR), oxazepam (OXA), temazepam (TEM), and venlafaxine (VEN) were purchased from Lipomed AG (Arlesheim, Switzerland). Deuterated derivatives of the analytes alprazolam-d5, diazepam-d5, flunitrazepam-d3, fluoxetine-d6, carbamazepine-d10, nordiazepam-d5, oxazepam-d5, and temazepam-d5, used as internal standards, were also purchased from Lipomed AG (Arlesheim, Switzerland). The selected number of 15 analytes, including six metabolites, was considered sufficient given that it was a preliminary study, and increasing the complexity of the method could potentially cause selectivity problems.
High-purity LC-MS chromatographic solvents acetonitrile, methanol, isopropyl alcohol, and sodium hydroxide obtained from Fluka Analytical (Seelze, Germany) were used in the experiment. Analytical-grade solvents acetonitrile, methanol, isopropyl alcohol, sodium hydroxide, and ammonium formate from Sigma-Aldrich (St. Louis, MO, USA) and formic acid obtained from Merck (Darmstadt, Germany) were also used. Deionized ultrapure water (18.2 M Ω · cm, TOC < 5 ppb) was obtained with the Milli-Q Plus system (Millipore, Bedford, MA, USA).

Wójtowicz A., Reciak M., Nowak P.M, & Wietecha-Posłuszny R. (2022). A sustainable approach for the stability study of psychotropic substances using vitreous humor and liver as alternative matrices. Analytical and Bioanalytical Chemistry, 414(21), 6355-6370.

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Analytical Workflow for Pharmaceuticals

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All solvents were of U(H)PLC grade. Merck KGaA supplied MeCN, and MeOH was purchased from Reuss-Chemie AG. Purified water was obtained from a Milli-Q integral water purification system.
Scharlau supplied DMSO, and formic acid was from BioSolve. Antipyrine and BSA were purchased from Sigma-Aldrich and Antipyrine-D3 from HPC Standards GmbH. Protopine HCl was purchased from
Extrasynthese SAS, and verapamil HCl from Sigma-Aldrich. Citalopram HBr, diazepam, and diazepam-D5 were purchased from Lipomed AG, and citalopram-D4 HBr was obtained from CDN Isotopes.

Spiess D., Abegg V.F., Chauveau A., Treyer A., Reinehr M., Oufir M., Duong E., Potterat O., Hamburger M, & Simões-Wüst A.P. (2022). Placental Passage of Protopine in an Ex Vivo Human Perfusion System. Planta Medica, 89(2), 194-207.

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Analytical Standards for Cannabis Compounds

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Analytical grade chloroform and methanol were from Dorwil (Buenos Aires, Argentina), HPLC grade acetonitrile was obtained from Carlo Erba (Val-de-Reuil, France), and formic acid, choline (> 98%), deuterochloroform (CDCl₃, 99.8%), and deuterium oxide (D₂O, 99.9%) were purchased from Merck (Kenilworth, NJ, USA). Betaine (> 98%) was from Thermo Fisher Scientific (Geel, Belgium). Diazepam (> 99.9%) and standard solutions of CBDA, CBD, cannabichromene (CBC), cannabinol (CBN), cannabigerol (CBG), THCA, THC, and Δ⁸-THC at 1 mg/mL were purchased from Lipomed (Cambridge, MA, USA). Standard solutions of Δ⁹-tetrahydrocannabivarinic acid (THCVA), Δ⁹-tetrahydrocannabivarin (THCV), cannabigerolic acid (CBGA), cannabidivarinic acid (CBDVA), cannabidivarin (CBDV) and cannabichromenic acid (CBCA) at 1 mg/mL were purchased from Cerilliant (Round Rock, TX, USA).

Fernández S., Castro R., López-Radcenco A., Rodriguez P., Carrera I., García-Carnelli C, & Moyna G. (2023). Beyond cannabinoids: Application of NMR-based metabolomics for the assessment of Cannabis sativa L. crop health. Frontiers in Plant Science, 14, 1025932.

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Quantitative Dried Blood Spot Analysis

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Methanol (MeOH) and acetonitrile (AcN), LC–MS grade, were purchased from HiPerSolv CHROMANORM^®. LC–MS grade isopropanol (IPA) was obtained from Fisher Scientific International, Inc., Hampton, NH, USA. A Milli-Q purification system (18.2 MΩ cm⁻¹) was used to provide ultrapure water. Ammonium formate (AF) ≥99% and formic acid (FA) 98–100% mobile phase additives were purchased from Riedel-de Haën^® (Sigma-Aldrich, Steinheim, Germany) and ChemLab, Zedelgem Belgium, respectively. Reference standards of diazepam, bromazepam, temazepam, oxazepam, alprazolam, 7-aminoflunitrazepam (7-AF), methadone, 3,4-methylenedioxymethamphetamine (MDMA), cathine, mescaline, cocaine, benzoylecgonine, methylecgonine, cocaethylene, 25B-NB2OMe, 25C-NB2OMe, 25I-NB2OMe, mephedrone, AH-7921, 1-Benzylpiperazine, methylone, 3,4-Methylenedioxypyrovalerone (MDPV), JWH-018, tetrahydrocannabinol (THC), cannabinol (CBN), and cannabidiol (CBD) were of more than 98% purity and were purchased from Lipomed AG (Arlesheim, Switzerland).
Dried blood spots (qDBSs) devices were obtained from Capitainer AB^® (Solna, Sweden).

Meikopoulos T., Gika H., Theodoridis G, & Begou O. (2024). Detection of 26 Drugs of Abuse and Metabolites in Quantitative Dried Blood Spots by Liquid Chromatography–Mass Spectrometry. Molecules, 29(5), 975.

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