Alk2 fc

Rabbit antibody against phospho-Smad1/5 (pSmad1/5, cat. No. 9516) was purchased from Cell Signalling Technology. Mouse monoclonal anti-α-tubulin (cat. No. T6199) was purchased from Sigma-Aldrich. Secondary anti-rabbit horseradish peroxidase (HRP) antibody (cat. No. P0448) and anti-mouse HRP antibody (cat. No. P0447) were both from Dako. Plasmid purification kits and RNA extraction kits were purchased from Qiagen. DMEM and CDCHO media were purchased from Thermo Fisher Scientific. Pulmonary artery endothelial cells (PAECs, cat. No. CC-2530) and endothelial growth medium (EGM2) were purchased from Lonza. HEK EBNA cells (cat. No. CRL-10852) and C2C12 cells (cat. No. CRL-1772) were purchased from ATCC. Chromatography columns were purchased from GE Healthcare. All crystallisation reagents were purchased from Hampton Research Inc and Molecular Dimensions. Human GF-domains of BMP2, BMP9 and BMP10, CV2, ALK1-Fc and ALK2-Fc were all purchased from R&D Systems.

ALK1-Fc, ALK2-Fc and ALK3-Fc were purchased from R&D Systems. Primary antibodies for western blot analysis were purchased from Cell Signalling Technology or CalBioreagents. All peptides used in this study were purchased and synthesised by PepMic (Suzhou, China). HPAECs and endothelial growth medium were purchased from Lonza (Wokingham, Berkshire, UK). HMEC-1 cells were from the Centre for Disease Control (CDC, Atlanta, GA). All other cell culture media were purchased from Life Technologies.