Infrared spectra of the solid samples were recorded on a PerkinElmer Spectrum-One FT-IR spectrophotometer by making KBr pellets. Powder X-ray diffraction patterns were recorded using a Bruker powder X-ray diffractometer D2 phaser. 1H-NMR spectra of complexes were recorded on a BRUKER Ascend-600 MHz NMR spectrometer using TMS as the internal standard. A PerkinElmer Lamda-750 spectrometer was used to record the solid-state UV-visible spectra by diffuse reflectance. Fluorescence emissions were measured in a Horiba Jobin Yvon Fluoromax-4 spectrofluorometer by taking definite amounts of solutions or definite amounts of solid samples and exciting at the required wavelength. Quantum yields were measured by taking definite amounts of solids and solutions and exciting at the required wavelength in a Horiba Jobin Yvon Fluoromax-4 spectrofluorometer by Petite integrating sphere method. The fluorescence emission (Ec) and the scatter (Lc) for the sample and blank (La and Ea) were recorded. From these spectral measurements (sample and blank), the quantum yields were calculated by using the equation φ = [(Ec − Ea)/(La − Lc)].
Jobin yvon fluoromax 4 spectrofluorometer
Manufactured by Horiba
Sourced in United States
The Jobin Yvon Fluoromax-4 is a spectrofluorometer designed for fluorescence analysis. It features excitation and emission monochromators, a photomultiplier tube detector, and advanced optics to measure the fluorescence properties of samples.
Automatically generated - may contain errors
Lab products found in correlation
Ascend 600 mhz nmr spectrometer, by Bruker (1 mentions)
Spectrum one ft ir spectrophotometer, by PerkinElmer (1 mentions)
D2 phaser powder x ray diffractometer, by Bruker (1 mentions)
Lamda 750 spectrometer, by PerkinElmer (1 mentions)
Advance x ray diffractometer, by Bruker (1 mentions)
Drx 400 spectrometer, by Bruker (1 mentions)
Liquid chromatography instrument, by Agilent Technologies (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
Evolution 600, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Bruker (1 mentions)
6 protocols using jobin yvon fluoromax 4 spectrofluorometer
1
Spectroscopic Characterization of Solid Complexes
2
Characterization of Crystalline Compounds
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All the chemicals and solvents were of reagent grade, purchased from different commercial sources and used without any further purification. Powder X-ray diffraction (PXRD) data were recorded on a Bruker Advance X-ray diffractometer with graphite monochromatized Cu Kα radiation (λ = 1.54056 Å) at room temperature (298 K). Range: 5–50°, step size: 0.02, number of steps: 2250 and time per step: 0.5 s. 1H NMR spectra were recorded on a 400 MHz Bruker DRX 400 spectrometer by calibrating the residual solvent as the reference in DMSO-d6 solution. The photoluminescence spectral measurements were conducted on a HORIBA Jobin Yvon FluoroMax-4 spectrofluorometer by packing the ground single crystals between the slides, which were placed vertically in the sample holder using λex = 310 nm. All UV-irradiation investigations were accomplished using a LUZCHEM UV reactor with the λex = 365 nm. Approximately 10 mg of powdered crystalline powder was packed between the glass slides and held vertically inside the photoreactor to ensure uniform exposure to UV radiation from both sides.
3
Luminescence Decay Measurements for q
4
Polymer Characterization by Multitechnique Analysis
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NMR
measurements were performed at room
temperature using a Bruker AVANCE III-500 MHz spectrometer instrument
in CDCl3 solvent. Size exclusion chromatography was performed
on an Agilent liquid chromatography instrument equipped with two similar
PL gel columns connected in series and two detectors, namely, a refractive
index detector and a UV–Vis detector. DMF was used as a mobile
phase (containing 0.005 M LiBr) at a flow rate of 1 mL min–1 at 45 °C, and the polymer concentration was 2 mg mL–1. The instrument was calibrated with polystyrene standards. UV–Vis
absorption spectra were recorded on a Thermo Evolution 600 UV–visible
spectrophotometer. Fluorescence emission spectra were recorded using
a Horiba Jobin Yvon Fluoromax-4 spectrofluorometer. Dynamic light
scattering (DLS) measurements were carried out at 25 °C using
a Malvern Zetasizer Nano ZS instrument equipped with a 30 mW He–Ne
laser light source emitting vertically polarized light of 632.8 nm
wavelength (scattering angle 173°). Transmission electron microscopy
images were recorded on an FEI-Technai Twin instrument operated at
120 kV in the bright-field mode. The aqueous solution of the sample
(1 mg mL–1) was drop-cast on a carbon-coated copper
grid followed by staining with uranyl acetate (2%).
measurements were performed at room
temperature using a Bruker AVANCE III-500 MHz spectrometer instrument
in CDCl3 solvent. Size exclusion chromatography was performed
on an Agilent liquid chromatography instrument equipped with two similar
PL gel columns connected in series and two detectors, namely, a refractive
index detector and a UV–Vis detector. DMF was used as a mobile
phase (containing 0.005 M LiBr) at a flow rate of 1 mL min–1 at 45 °C, and the polymer concentration was 2 mg mL–1. The instrument was calibrated with polystyrene standards. UV–Vis
absorption spectra were recorded on a Thermo Evolution 600 UV–visible
spectrophotometer. Fluorescence emission spectra were recorded using
a Horiba Jobin Yvon Fluoromax-4 spectrofluorometer. Dynamic light
scattering (DLS) measurements were carried out at 25 °C using
a Malvern Zetasizer Nano ZS instrument equipped with a 30 mW He–Ne
laser light source emitting vertically polarized light of 632.8 nm
wavelength (scattering angle 173°). Transmission electron microscopy
images were recorded on an FEI-Technai Twin instrument operated at
120 kV in the bright-field mode. The aqueous solution of the sample
(1 mg mL–1) was drop-cast on a carbon-coated copper
grid followed by staining with uranyl acetate (2%).
5
Spectrofluorimetric Analysis of EVs
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EVs were lysated in
the presence of triton 0.1% in PBS and analyzed by spectrofluorimetry
(HORIBA Jobin Yvon Fluoromax-4 spectrofluorometer, Edison, NY, USA).
The nanomoles of FITC calculated for each EV samples were normalized
to mg of total EVs proteins previously determined by the Bradford
assay. EVs derived from not treated MSCs were used as control in all
the performed studies.
the presence of triton 0.1% in PBS and analyzed by spectrofluorimetry
(HORIBA Jobin Yvon Fluoromax-4 spectrofluorometer, Edison, NY, USA).
The nanomoles of FITC calculated for each EV samples were normalized
to mg of total EVs proteins previously determined by the Bradford
assay. EVs derived from not treated MSCs were used as control in all
the performed studies.
6
Structural and Spectroscopic Characterization
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The X-ray diffraction powder patterns were recorded at room temperature on a Bruker D8 Advance (SeriesII-2009) with Cu Kα radiation on the 10–80° 2θ range with operating at 45 kV and 40 mA. Fourier transforms infrared spectroscopy (FTIR) for the titled compounds were recorded at room temperature on Perkin Elmer Spectrum 1000 FT-IR spectrometer in the 400–1400 cm−1 range. The photoluminescence excitation (PLE) and photoluminescence (PL) spectra were recorded on a Jobin Yvon Fluoromax-4 Spectrofluorometer (Horiba, USA) equipped with a 150 W Xe lamp used as an excitation source.
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