The experimental design was performed as described in previous studies with some modifications (Li et al., 2017 (link)). Fifty male SD rats were randomly divided into five groups (n = 10) and orally administered an equal 10 ml/kg dose of a normal saline solution (the control group and model group), 10 mg/kg allopurinol (Macklin Biochemical Technology Co., Ltd., China) (the positive control group), 150 mg/kg polyphenols from wild P. igniarius (the WPP-treated group) and 150 mg/kg polyphenols from cultivated P. igniarius (the CPP-treated group) for 7 days. Yeast extract (Beyotime Biochemical Technology Co., Ltd., China) (10 g/kg) and adenine (Macklin Biochemical Technology Co., Ltd., China) (100 mg/kg) were gavaged 12 h prior to allopurinol, WPP, and CPP administration every day except for the control group. The model group, positive control group, WPP-treated group and CPP-treated group were intraperitoneally injected with potassium oxonate (Yuanye Biochemical Technology Co., Ltd., China) (300 mg/kg) on days 1, 3, and 7, while the control group was injected with an equal volume of vehicle (0.5% CMC-Na).
Adenine
Manufactured by Maclin Biochemical Technology
Sourced in China
Adenine is a purine-based organic compound that serves as a fundamental building block of nucleic acids, such as DNA and RNA. It plays a crucial role in various cellular processes and metabolic pathways. As a core component of nucleic acids, adenine is essential for the storage and transmission of genetic information within living organisms.
Automatically generated - may contain errors
Lab products found in correlation
Potassium oxonate, by Maclin Biochemical Technology (2 mentions)
Allopurinol, by Maclin Biochemical Technology (1 mentions)
Male sprague dawley rats, by Jackson ImmunoResearch (1 mentions)
Potassium monopersulfate triple salt, by Maclin Biochemical Technology (1 mentions)
Spectrum one ft ir spectrophotometer, by PerkinElmer (1 mentions)
Jsm 6510lv scanning electron microscope, by JEOL (1 mentions)
Copper acetate monohydrate, by Maclin Biochemical Technology (1 mentions)
D8 advance x ray diffractometer, by Bruker (1 mentions)
Disodium tetraborate decahydrate, by Sinopharm (1 mentions)
Sodium carboxymethyl cellulose, by Sinopharm (1 mentions)
Acetic acid, by Sinopharm (1 mentions)
Hplc grade methanol, by Tedia (1 mentions)
Perchloric acid, by Sinopharm (1 mentions)
Boric acid, by Sinopharm (1 mentions)
Sodium carboxymethyl cellulose, by Maclin Biochemical Technology (1 mentions)
5 protocols using adenine
1
Polyphenol Modulation of Hyperuricemia in Rats
2
Establishing Adenine-Induced Chronic Kidney Disease Rat Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male Sprague Dawley rats (8 weeks old; 250 g; Jackson Laboratory, Maine, USA) were reared with a 12 h light/dark cycle. Rats were orally administered with adenine (0.1 g/kg) and potassium oxalate (1.5 g/kg, Macklin, Shanghai, China) every day to establish a HN rat model for a total of 3 weeks [1 (link)]. Then, rats were injected with 100 μL HDAC3 low-expression vector negative control (sh-NC), HDAC3 low-expression vector (sh-HDAC3), miR-19b-3p mimic NC, miR-19b-3p mimic, or sh-HDAC3 and miR-19b-3p-inhibitor. The injection was performed twice a week, for 6 weeks [16 (link)].
3
Synthesis and Characterization of Metal-Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Copper acetate monohydrate (C4H6CuO4·H2O), cobalt(iii ) acetate tetrahydrate (C4H6CoO4·4H2O), potassium monopersulfate triple salt (42–46% KHSO5 basis), and adenine were purchased from Shanghai Macklin Biochemical Co., Ltd. Doxorubicin (DOX) and absolute ethanol were purchased from Aladdin Chemistry, Co. (Shanghai) and used as received. N,N-dimethylformamide (C3H7NO, DMF) was purchased from Sinopharm Chemical Reagent Co., Ltd. Aqueous solutions were prepared with deionized water. All other chemicals used in this work were of analytical grade, obtained from commercial suppliers, and used without further purification unless otherwise noted.
The powder X-ray diffractometry (XRD) patterns were obtained using a D8 advance X-ray diffractometer (Bruker Company, Germany). Fourier transform infra-red (FT-IR) spectra were obtained using a spectrum one FT-IR spectrophotometer (PerkinElmer, USA) at room temperature. Morphologies of the samples were observed on a JSM6510LV scanning electron microscope (SEM, JEOL, Japan). The elemental composition of the sample was analyzed using energy dispersive X-ray spectroscopy (EDX) and elemental mapping in a Super-XTM system.
The powder X-ray diffractometry (XRD) patterns were obtained using a D8 advance X-ray diffractometer (Bruker Company, Germany). Fourier transform infra-red (FT-IR) spectra were obtained using a spectrum one FT-IR spectrophotometer (PerkinElmer, USA) at room temperature. Morphologies of the samples were observed on a JSM6510LV scanning electron microscope (SEM, JEOL, Japan). The elemental composition of the sample was analyzed using energy dispersive X-ray spectroscopy (EDX) and elemental mapping in a Super-XTM system.
4
Extraction and Quantification of Allopurinol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Allopurinol tablets were procured from Shimao Tianjie Pharmaceutical (Jiangsu) Co., Ltd. Carboxymethylcellulose sodium, boric acid (Guaranteed reagent), disodium tetraborate decahydrate (Analytical Reagent), perchloric acid (Analytical Reagent), and acetic acid (Analytical Reagent) were all acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Potassium oxonate and adenine were sourced from Shanghai Macklin Biochemical Technology Co., Ltd. Yeast extract and 0.9% sodium chloride injection were purchased from Shanghai Chunshi Biotechnology Co., Ltd. (Shanghai, China) and Kelun Pharmaceutical Co., Ltd. (Sichuan, China), respectively. High-performance liquid chromatography (HPLC) grade methanol was obtained from TEDIA Company Inc (USA).
5
Hyperuricemia Induction in Sprague-Dawley Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TSPCs were isolated from AT tissue of Sprague-Dawley rats (male = 6, female = 6; 8 weeks) as previously described [23 (link)]. The protocol for the use of rats was approved by the Institutional Animal Care and Use Committee (IACUC) of Chongqing Medical University. For the hyperuricemia rat model, 8-week-old Sprague-Dawley rats were assigned to the hyperuricemia group (n = 12) and the control group (n = 12). Rats in the hyperuricemia group were intragastrically administered with adenine (0.1 grams/kilogram, Macklin, Shanghai, China) and potassium oxonate (1.5 grams/kilogram, Macklin) dissolved in 0.5% sodium carboxymethylcellulose (Macklin) once per day for 6 successive weeks. On the same days, rats in the control group were intragastrically administered with equal volumes of 0.5% sodium carboxymethylcellulose only.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!