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The OVCA432 is a cell line derived from human ovarian carcinoma. It is a widely used model for the study of ovarian cancer biology and the evaluation of potential therapeutic agents. The OVCA432 cell line is available for purchase from the American Type Culture Collection (ATCC) for research purposes.

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Lab products found in correlation

Skov3, by American Type Culture Collection (5 mentions) Ovcar3, by American Type Culture Collection (4 mentions) A2780, by American Type Culture Collection (3 mentions) Mda mb 231, by American Type Culture Collection (2 mentions) Rnaimax, by Thermo Fisher Scientific (2 mentions) Mcf 7, by American Type Culture Collection (2 mentions) Heya8, by American Type Culture Collection (2 mentions) Gentamicin sulfate, by Gemini Bio (2 mentions) Rpmi 1640, by Gemini Bio (2 mentions) Igrov, by American Type Culture Collection (2 mentions) Gilm2, by American Type Culture Collection (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

5 protocols using ovca432

1

Isolation and culture of ovarian cancer cells

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Sample collection was performed with patient consent and approved by the Human Investigation Committee of Yale University School of Medicine. Ovarian cancer cells were isolated from patients diagnosed with Stage III/IV serous ovarian carcinoma. These in-house derived ovarian cancer cells (R182, R2615, 01-28, and Tara R182) have been previously described [10 (link),11 (link),12 (link),13 (link),14 (link),15 (link)]. Ovarian cancer cell lines (OVCAR3, OVCA432, SKOV3, and A2780) were purchased from ATCC. Cells were cultured in RPMI media supplemented in 10% FBS and grown at 37 °C with 5% CO2. All of the cell lines tested negative for mycoplasma, authenticated once a year by STR profiling, and used within six passages between experiments.
Tedja R., Alvero A.B., Fox A., Cardenas C., Pitruzzello M., Chehade H., Bawa T., Adzibolosu N., Gogoi R, & Mor G. (2023). Generation of Stable Epithelial–Mesenchymal Hybrid Cancer Cells with Tumorigenic Potential. Cancers, 15(3), 684.
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2

Cell Line Characterization and Tumor Sample

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HEY and OC316 cell lines were obtained from Robert C. Bast lab in MD Anderson Cancer Centre. SKOV3 and OVCA432 cell lines were purchased from ATCC. All cells were cultured in RPMI1640 medium (Invitrogen), supplemented with 10% fetal bovine serum (Gibco), 1% penicillin-streptomycin (Gibco). The cell lines have been tested and confirmed without mycoplasma contamination. Fresh-frozen tumour sample was from the patient, who provided written informed consent for participation in the prospective biomarker validation study Gynecological Oncology Targeted Therapy Study 01 (GO-Target-01), under research ethics approval 11/SC/0014 by the South Central Berkshire Research Ethics Committee.
Wu W., Xing X., Wang M., Feng Y., Wietek N., Chong K., El-Sahhar S., Ahmed A.A., Zang R, & Zheng Y. (2022). Investigation of the Potential Mechanisms Underlying Nuclear F-Actin Organization in Ovarian Cancer Cells by High-Throughput Screening in Combination With Deep Learning. Frontiers in Cell and Developmental Biology, 10, 869531.
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3

Ovarian Cancer Cell Isolation and Characterization

Cited 1 time
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Sample collection was performed with patient consent and approved by the Human Investigation Committee of Yale University School of Medicine. Ovarian cancer cells were isolated from patients diagnosed with stage III/IV serous ovarian carcinoma. These in-house derived ovarian cancer cells (R182, R2615, OCSC1-F2, 01–28, and Tara R182) have been previously described (30 (link),37 (link)–40 (link)). Ovarian cancer cell lines (OVCAR3, OVCA432, SKOV3) were purchased from ATCC. Cells were cultured in RPMI media supplemented in 10% FBS and grown at 37oC with 5% CO2. All the cell lines have been tested negative for mycoplasma, authenticated in May 2016 by STR profiling, and used within 6 passages between experiments.
Sample collection from various ovarian and intra-peritoneal cancers was also carried out with patient consent and approved by the Human Investigations Committee of Yale University School of Medicine. Tumor tissues were flash-frozen and stored in liquid nitrogen until protein was extracted by homogenization.
Yang-Hartwich Y., Tedja R., Roberts C., Goodner-Bingham J., Cardenas C., Gurea M., Sumi N.J., Alvero A.B., Glackin C.A, & Mor G. (2018). p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Molecular cancer research : MCR, 17(1), 153-164.
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4

Protocols for Culturing and Transfecting Ovarian and Breast Cancer Cell Lines

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All cell lines were maintained in 5% CO2 at 37°C. Ovarian cancer (A2780, OVCAR3, SKOV3, OVCA432, HeyA8, IGROV, EG) and breast cancer (MDA-MB-231, MCF7, GILM2) cells were obtained from the American Type Culture Collection and were maintained in RPMI 1640 supplemented with 10–15% fetal bovine serum (FBS) and 0.1% gentamicin sulfate (GeminiBioproducts, Calabasas, CA). All cell lines were routinely tested to confirm the absence of Mycoplasma, and all in vitro experiments were conducted with 60–80% confluent cultures.
All siRNA transfections (Supplementary Table 1) were performed using RNAi MAX (Invitrogen Carlsbad, CA) reagent using forward transfection protocol from the manufacturer. Media was changed 5 hours after transfections to minimize toxicity. For all hypoxia treatments, cells were incubated in an oxygen-controlled hypoxia chamber at 1% O2. For ectopic expression of Drosha and Dicer, we obtained plasmids from Addgene (IDs 10828, 25851 respectively). Next, we cloned open reading frames into pLKO.1-GFP or Puromycin lentiviral plasmids. We transduced HeyA8 cells with virus particles and then selection using GFP (Drosha) or puromycin (Dicer) was carried out to establish stable cell variants.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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5

Protocols for Culturing and Transfecting Ovarian and Breast Cancer Cell Lines

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All cell lines were maintained in 5% CO2 at 37°C. Ovarian cancer (A2780, OVCAR3, SKOV3, OVCA432, HeyA8, IGROV, EG) and breast cancer (MDA-MB-231, MCF7, GILM2) cells were obtained from the American Type Culture Collection and were maintained in RPMI 1640 supplemented with 10–15% fetal bovine serum (FBS) and 0.1% gentamicin sulfate (GeminiBioproducts, Calabasas, CA). All cell lines were routinely tested to confirm the absence of Mycoplasma, and all in vitro experiments were conducted with 60–80% confluent cultures.
All siRNA transfections (Supplementary Table 1) were performed using RNAi MAX (Invitrogen Carlsbad, CA) reagent using forward transfection protocol from the manufacturer. Media was changed 5 hours after transfections to minimize toxicity. For all hypoxia treatments, cells were incubated in an oxygen-controlled hypoxia chamber at 1% O2. For ectopic expression of Drosha and Dicer, we obtained plasmids from Addgene (IDs 10828, 25851 respectively). Next, we cloned open reading frames into pLKO.1-GFP or Puromycin lentiviral plasmids. We transduced HeyA8 cells with virus particles and then selection using GFP (Drosha) or puromycin (Dicer) was carried out to establish stable cell variants.
Rupaimoole R., Wu S.Y., Pradeep S., Ivan C., Pecot C.V., Gharpure K.M., Nagaraja A.S., Armaiz-Pena G.N., McGuire M., Zand B., Dalton H.J., Filant J., Miller J.B., Lu C., Sadaoui N.C., Mangala L.S., Taylor M., van den Beucken T., Koch E., Rodriguez-Aguayo C., Huang L., Bar-Eli M., Wouters B.G., Radovich M., Ivan M., Calin G.A., Zhang W., Lopez-Berestein G, & Sood A.K. (2014). Hypoxia Mediated Downregulation of miRNA Biogenesis Promotes Tumor Progression. Nature communications, 5, 5202.
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