Anti stat5b

Manufactured by Santa Cruz Biotechnology

Anti-STAT5B is a laboratory reagent used in scientific research. It is a highly specific antibody that binds to the STAT5B protein, a transcription factor involved in cellular signaling pathways. This product can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the STAT5B protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti brdu clone b44, by BD (1 mentions) Anti stat5a, by Santa Cruz Biotechnology (1 mentions) Rabbit anti stat5, by Santa Cruz Biotechnology (1 mentions) Anti atm ps1981, by Abcam (1 mentions) Anti atr pt1989, by GeneTex (1 mentions) Anti mcm2, by Cell Signaling Technology (1 mentions) Anti pstat5 y694, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Ecl plus detection kit, by GE Healthcare (1 mentions) Goat anti rabbit immunoglobulin g, by Santa Cruz Biotechnology (1 mentions) Hybond n membrane, by GE Healthcare (1 mentions)

4 protocols using anti stat5b

STAT5B Binding to Cis Promoter

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIPs were performed as previously described (Jensik and Arbogast, 2011 (link)). Briefly, stable PRLR-HA CAD
cells were treated with vehicle or rPRL for 1 hr prior to cross-linking with
formaldehyde. ChIP was performed using 1 µg Anti-STAT5B (Santa Cruz
Biotechnology; G-2) or normal mouse immunoglobulin (mIgG; Santa Cruz
Biotechnology). ChIP samples were analyzed by PCR using mouse
Cis −184 and +4200 PCR primer sets (Basham et al., 2008 (link)). PCR products were
separated on acrylamide gels and stained with ethidium bromide.

Jensik P.J, & Arbogast L.A. (2014). Regulation of cytokine-inducible SH2-containing protein (CIS) by ubiquitination and Elongin B/C interaction. Molecular and cellular endocrinology, 401, 130-141.

+ Open protocol

+ Expand

Comprehensive Antibody Inventory for Immunochemical Analyses

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following primary antibodies were purchased: mouse anti-STAT5 (cat# sc-74442), rabbit anti-STAT5 (cat# sc-835), anti-STAT5A (cat# sc-271542) and anti-STAT5B (cat# sc-1656), anti-BrdU (IIB5) (cat# sc-32323) were from Santa Cruz (Dallas, TX); anti-MCM2 (cat# 12079), and anti-pSTAT5(Y694) (cat# 4322) were from Cell Signaling (Danvers, MA); anti-STAT5A/b pan-specific antibody (cat # AF2168) and normal IgG rabbit (cat# AB-105-C) were from R&D Systems Inc (Minneapolis, MN); anti-MCM5 antibody (cat# 2380–1) was from Epitomics (Burlingame, CA); anti-B19V capsid (cat# Mab8293) was from Millipore (Billerica, MA); anti-BrdU (clone B44) was from BD (Franklin Lakes, NJ); and anti-β-actin (cat# A5441) was from Sigma; anti-MCM3 (cat# A300-124A), anti-MCM5 (cat# A300-195A; for ChIP), and MCM7(cat#A300-128A) were from Bethyl Laboratories (Montgomery, TX); anti-ATM(pS1981) (cat#ab81292) were from Abcam (Cambridge, MA); and anti-ATR(pT1989) (cat#GTX128145) from GeneTex (Irvine, CA). Rat anti-NS1 polyclonal antibody was prepared in our lab as previously reported [25 (link)].
Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Sigma, and fluorescein isothiocyanate (FITC)-, Texas Red-, and Dylight405-conjugated anti-mouse, anti-rat, and anti-rabbit secondary antibodies were all purchased from Jackson ImmunoResearch (West Grove, PA).

Ganaie S.S., Zou W., Xu P., Deng X., Kleiboeker S, & Qiu J. (2017). Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex. PLoS Pathogens, 13(5), e1006370.

+ Open protocol

+ Expand

Verifying STAT5b Expression in DF-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To verify STAT5b expression in DF-1 cells after transfection, Western blotting was performed. Whole-cell lysates were made from both untransfected DF-1 cell cultures and DF-1 cells transfected with either RCAS-STAT5b or RCAS-PDGFB. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to fractionate 10-µg protein samples using gels with 10% polyacrylamide. Samples were transferred to a polyvinylidene difluoride membrane and probed with anti-influenza hemagglutinin antibody (1:1000; F7; Santa Cruz Biotechnology, Santa Cruz, CA) to verify PDGFB expression and with anti-STAT5b (1:1000, Santa Cruz Biotechnology) to detect total STAT5b expression. Goat anti-rabbit immunoglobulin G (1:2500; Santa Cruz Biotechnology) was used as the secondary antibody. The ECL Plus detection kit (GE Healthcare, Waukesha, WI) was used per the manufacturer’s instructions to develop the blots.

Gressot L.V., Doucette T.A., Yang Y., Fuller G.N., Heimberger A.B., Bögler O., Rao A., Latha K, & Rao G. (2014). Signal transducer and activator of transcription 5b drives malignant progression in a PDGFB-dependent proneural glioma model by suppressing apoptosis. International journal of cancer. Journal international du cancer, 136(9), 2047-2054.

+ Open protocol

+ Expand

Electrophoretic Mobility Shift Assay for c-Jun

Check if the same lab product or an alternative is used in the 5 most similar protocols

EMSA was performed as described [28 (link)]. Nuclear extracts of the control and c-Jun expression plasmid transfected HeLa cells were added to binding buffer, and the mixture was incubated for 10 min at 25°C. Digoxigenin-labeled double-stranded oligonucleotide (Table 1) was added in the absence or presence of an unlabeled competitor, and the binding mixture was incubated for 30 min at 25°C. For some experiments, anti-c-JUN or anti-STAT5B antibodies (Santa Cruz Biotechnology, Inc. Santa Cruz, CA) were added and incubated for 10 min. The mixture was kept on ice for 10 min and loaded onto the 4% polyacrylamide gels in 0.5x TBE. The gel was run in 0.5x TBE buffer at 100 V for 70 min. After electrophoresis, the gel was transferred onto a Hybond-N+ membrane (GE Healthcare UK Ltd.) and cross-linked by UV. Detection was performed as described [28 (link)].

Nakata T., Toyoshima Y., Yagi T., Katsumata H., Tokita R, & Minami S. (2020). Growth hormone increases regulator of calcineurin 1-4 (Rcan1-4) mRNA through c-JUN in rat liver. PLoS ONE, 15(6), e0235270.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!