Red imaging system

Manufactured by Bio-Techne

Sourced in United States

The Red Imaging system is a laboratory equipment designed for high-quality imaging and documentation of gels, blots, and other samples. It features a sensitive camera, adjustable lighting, and software for image acquisition and analysis.

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Lab products found in correlation

5 fluoroorotic acid 5 foa, by GoldBio (1 mentions) 1 kb ladder, by New England Biolabs (1 mentions) Zyppy plasmid miniprep kit, by Zymo Research (1 mentions) Redsafe, by iNtRON Biotechnology (1 mentions) Pc707, by Astec (1 mentions)

3 protocols using red imaging system

Yeast Growth Media Preparation and Transformation

Cited 1 time

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Yeast growth media, including YPDA, YPG and synthetic dropout media, were
prepared as described [51 (link)].
Transformation of DNA into yeast cells was performed using early stationary
phase cells and a PEG/LiAc chemical-based method [52 (link)]. Synthetic media plates containing 2% glucose and
1% 5-fluoroorotic acid (5-FOA) (Gold Biotechnology) were used for
counterselective growth of Ura⁻ cells. Plasmid DNAs were
extracted from E. coli DH5a cells grown in liquid TB broth
using Qiaprep plasmid miniprep kits. Horizon 11–14 gel rigs were used to
perform gel electrophoresis using 0.7% - 0.9% agarose gels in 1 X TAE buffer (40
mM Tris, 20 mM acetic acid, 1 mM EDTA). Gels were stained in ethidium bromide
(0.5 μg/mL) for 15–20 min and images were captured using an Alpha
Innotech Red Imaging system.

Ghanem N.Z., Malla S.R., Araki N, & Lewis L.K. (2019). Quantitative assessment of changes in cell growth, size and morphology during telomere-initiated cellular senescence in Saccharomyces cerevisiae. Experimental cell research, 381(1), 18-28.

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Isolation and Characterization of Plasmids from Francisella

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pFNMB1 msfgfp and pFNMB2 msfgfp were recovered from F. novicida with a Zyppy Plasmid Miniprep Kit (Zymo Research) after passaging the cultures for 4 days in liquid BHI supplemented with 100 μg/ml ampicillin and 500 ng/ml ATc as described above. About 250 ng of each plasmid DNA was then transformed into chemo-competent E. coli DH5α λpir. The transformed E. coli were plated on LB agar plates supplemented with 50 μg/ml kanamycin. Three independent experiments were carried out. The next day, colonies were grown in liquid LB supplemented with 50 μg/ml kanamycin, plasmid DNA was isolated and 250 ng of each plasmid was digested with SacI-HF and SpeI restriction enzymes (New England BioLabs) for 1 h. As control, both plasmids were additionally isolated from E. coli directly, without passaging in F. novicida, and digested identically. After heat inactivation of the enzymes (80°C for 20 min), the digested plasmids were loaded on a 1% agarose gel (BioConcept) together with a 1 kb ladder (New England BioLabs). DNA was stained with RedSafe (iNtRON Biotechnology) and a Red imaging system (Alpha Innotech) was used for imaging.

Brodmann M., Heilig R., Broz P, & Basler M. (2018). Mobilizable Plasmids for Tunable Gene Expression in Francisella novicida. Frontiers in Cellular and Infection Microbiology, 8, 284.

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Quantitative RT-PCR Analysis in Zebrafish

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Total RNA was isolated from whole zebrafish embryos using QIAzol Lysis Reagent (Cat. No. 79306; Qiagen, Venlo, Netherlands). The RT reaction was performed with 300 ng of total RNA prepared from each sample using an Omniscript RT Kit (Ca. No. 205111; Qiagen) with a mixture of oligo(dT) and random primers. The RT product (1 µL) was subjected to PCR amplification (15 µL) using a Taq PCR Core Kit (Ca. No. 201223; Qiagen) in a standard thermal cycler PC707 (ASTEC, Fukuoka, Japan). Thermal cycling conditions were as follows: 94 °C for 30 s, 53 °C for 30 s, and 72 °C for 60 s.
The following primers were used: vegfaa, fwd 5'-CTCCTCCATCTGTCTGCTGTAAAG-3' and rev 5'-CTCTCTGAGCAAGGCTCACAG-3' (29 cycles, product size: 490 bp); nos2a, fwd 5'-GTGTTCCCTC AGAGAACAGAT-3' and rev 5'-GATCAGTCCTTTGAAGCTGAC-3' (35 cycles, product size: 822 bp); and elfa, fwd 5'-CTTCTCAGGCTGACTGTGC-3' and rev 5'-CCGCTAGCATTACCCTCC-3' (29 cycles, product size: 358 bp). PCR products were separated by electrophoresis on a 1.5% agarose gel and visualized with SYBR Gold Nucleic Acid Gel Stain (Cat. No. S11494; Molecular Probes, Eugene, OR, USA), followed by detection under a UV transilluminator (Red Imaging System; Alpha Innotech, San Diego, CA, USA). PCR bands were quantitated by densitometry using the ImageJ software in a blinded manner and normalized to the levels of elfa.

Tsuji-Tamura K., Sato M., Fujita M, & Tamura M. (2020). Glycine exerts dose-dependent biphasic effects on vascular development of zebrafish embryos. Biochemical and biophysical research communications, 527(2).

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