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Gsk lsd1 2hcl

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GSK-LSD1 2HCL is a chemical compound used in laboratory research. It serves as a selective inhibitor of the enzyme lysine-specific demethylase 1 (LSD1). This compound can be utilized in various experimental settings to study the role of LSD1 in biological processes.

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Lab products found in correlation

Sp2509, by Selleck Chemicals (2 mentions) Pargyline, by Yuanye Bio-Technology (2 mentions) Chloroquine diphosphate, by Merck Group (1 mentions) Bafilomycin a1, by Selleck Chemicals (1 mentions) Dmem f12 basic medium, by Thermo Fisher Scientific (1 mentions) 6 well culture dishes, by NEST Biotechnology (1 mentions) Cycloheximide hy 12320, by MedChemExpress (1 mentions) Z vad fmk, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions) Gsk j4, by Merck Group (1 mentions) Ory 1001, by Selleck Chemicals (1 mentions) Tamoxifen, by Merck Group (1 mentions)

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GSK-LSD1 (6 citations)

4 protocols using gsk lsd1 2hcl

1

Ovarian Tissue Culture with Inhibitors

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Ovaries were separated by microdissection from the mesonephros or ovarian capsule in prechilled PBS (10 mM, pH 7.4) under a stereomicroscope (ZSA302, COIC). The isolated ovaries were cultured in 6‐well culture dishes (NEST Biotechnology) with basic DMEM/F‐12 medium (Gibco, Life Technologies) and Penicillin‐Streptomycin at 37°C in a 5% CO2, 95% air atmosphere with saturated humidity.
Ovaries were cultured in either medium with dimethylsulfoxide (DMSO) or medium supplemented with inhibitor. The concentration of inhibitors used in this study: GSK‐LSD1‐2Hcl (S7574; Selleck): 64 μM; 3MA (S2767; Selleck, USA): 2.5 mM; Z‐VAD‐fmk (S7023; Selleck): 50 μM; Chloroquine diphosphate (C6688; Sigma): 10 μM; Bafilomycin A1 (S1413; Selleck): 60 nM; MG132 (S2619; Selleck): 5 μM; Cycloheximide (HY‐12320; MedChemExpress): 2 nM; and SP2509 (S7680; Selleck): 5 nM.
He M., Zhang T., Zhu Z., Qin S., Wang H., Zhao L., Zhang X., Hu J., Wen J., Cai H., Xin Q., Guo Q., Lin L., Zhou B., Zhang H., Xia G, & Wang C. (2020). LSD1 contributes to programmed oocyte death by regulating the transcription of autophagy adaptor SQSTM1/p62. Aging Cell, 19(3), e13102.
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2

LSD1 Inhibitors Modulate Cell Viability

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LSD1 inhibitors GSK-LSD1 2HCL [Selleck (Houston, TX), S7574] and pargyline [Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China), C127543] were used to treat cells. Cells were seeded in 96-well plate at 500 cells/well, and treated with GSK LSD1 2HCL at 50 nm or paragyline at 50 μm for 24, 48 and 72 hours. Cell viabilities were analyzed with the MTT assay.
Hu X., Xiang D., Xie Y., Tao L., Zhang Y., Jin Y., Pinello L., Wan Y., Yuan G.C, & Li Z. (2019). LSD1 suppresses invasion, migration and metastasis of luminal breast cancer cells via activation of GATA3 and repression of TRIM37 expression. Oncogene, 38(44), 7017-7034.
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3

LSD1 Inhibitors Modulate Cell Viability

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LSD1 inhibitors GSK-LSD1 2HCL [Selleck (Houston, TX), S7574] and pargyline [Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China), C127543] were used to treat cells. Cells were seeded in 96-well plate at 500 cells/well, and treated with GSK LSD1 2HCL at 50 nm or paragyline at 50 μm for 24, 48 and 72 hours. Cell viabilities were analyzed with the MTT assay.
Hu X., Xiang D., Xie Y., Tao L., Zhang Y., Jin Y., Pinello L., Wan Y., Yuan G.C, & Li Z. (2019). LSD1 suppresses invasion, migration and metastasis of luminal breast cancer cells via activation of GATA3 and repression of TRIM37 expression. Oncogene, 38(44), 7017-7034.
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4

Characterization of LSD1 Inhibitor Compounds

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GSK-LSD1 2HCl, SP2509, ORY-1001, and GSK2879552 2HCl compounds were purchased from Selleckchem (Huston, USA); tamoxifen, TCP, and GSK-J4 from Sigma-Aldrich (St Louis, USA); SAHA from Merck (Kenilworth, N.J., U.S.A). MC3324 was synthesized by Prof. Mai’s group (“Sapienza” University of Rome), as reported in [18 (link)]. The MC3324 derivatives MC4379, MC4380, and MC4266 were synthesized as reported in supplementary materials. Compounds were used at concentrations indicated in figures or legends.
Benedetti R., Dell’Aversana C., De Marchi T., Rotili D., Liu N.Q., Novakovic B., Boccella S., Di Maro S., Cosconati S., Baldi A., Niméus E., Schultz J., Höglund U., Maione S., Papulino C., Chianese U., Iovino F., Federico A., Mai A., Stunnenberg H.G., Nebbioso A, & Altucci L. (2019). Inhibition of Histone Demethylases LSD1 and UTX Regulates ERα Signaling in Breast Cancer. Cancers, 11(12), 2027.
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