This project was reviewed and approved by the Institutional Review Board of the United Arab Emirates University, College of Medicine and Health Sciences, and experiments were performed in accordance with protocols approved by the Institutional Animal Care and Research Advisory Committee.
Both male and female BALB/C mice (body weight: 23 ± 2 g) (Taconic Farms Inc., Germantown, NY, USA) were housed in light (12 h light : 12 h dark cycle) and temperature-controlled (22 ± 1°C) rooms. They had free access to commercial laboratory chow and were provided tap water ad libitum.
Mice were anesthetized with sodium pentobarbital (60 mg/kg, i.p.) and placed supine with extended neck on an angled board. A Becton Dickinson 24 Gauge cannula was inserted via the mouth into the trachea. The CeO2 NPs suspensions (0.5 mg/kg) or saline-only were instilled intratracheally (i.t.) (100 μL) via a sterile syringe and followed by an air bolus of 100 μL, and 24 h later several endpoints were measured (Figure 1 ). The experiments were repeated three times.
Both male and female BALB/C mice (body weight: 23 ± 2 g) (Taconic Farms Inc., Germantown, NY, USA) were housed in light (12 h light : 12 h dark cycle) and temperature-controlled (22 ± 1°C) rooms. They had free access to commercial laboratory chow and were provided tap water ad libitum.
Mice were anesthetized with sodium pentobarbital (60 mg/kg, i.p.) and placed supine with extended neck on an angled board. A Becton Dickinson 24 Gauge cannula was inserted via the mouth into the trachea. The CeO2 NPs suspensions (0.5 mg/kg) or saline-only were instilled intratracheally (i.t.) (100 μL) via a sterile syringe and followed by an air bolus of 100 μL, and 24 h later several endpoints were measured (