L ascrobic acid

Drugs and reagents were obtained from the following sources: 3-(3-carbamoylphenyl)phenyl N-cyclohexylcarbamate (URB597), 8-iso Prostaglandin F_2α-d₄ (8-isoPGF2α–d₄), 8-iso Prostaglandin F_2α (8-isoPGF2α) anandamide-d₈ (AEA-d₈), and 2-arachidonylglycerol-d₈ (2-AG-d₈) from Cayman Chemical Company (Ann Arbor, MI, USA); dimethyl sulfoxide (DMSO), superoxide dismutase (SOD), catalase (CAT), L-ascrobic acid, retinol, L-glutathione reduced (GSH), and Tween 80 were acquired from Sigma-Aldrich (Steinheim, Germany); pentobarbital sodium was purchased from Biowet (Puławy, Poland), chloro-2,4-dinitro benzene (CDNB), butylated hydroxytoluene (BHT), and 5,5′-dithiobis (2-dinitrobenzoic acid) (DTNB) were acquired from Sigma-Aldrich (Steinheim, Germany). URB597 was dissolved in an URB597 solvent: a mixture of DMSO, Tween 80, and saline (0.9% NaCl) [1:2:7; v/v/v].

Drugs and reagents were obtained from the following sources: 3-(3-carbamoylphenyl)phenyl N-cyclohexylcarbamate (URB597), 4-hyroxynonenal (4-HNE), 8-iso Prostaglandin F_2α-d₄ (8-isoPGF2α–d₄), 8-iso Prostaglandin F_2α (8-isoPGF2α) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from Cayman Chemical Company (Ann Arbor, MI, USA); 11-deoxycorticosterone acetate (DOCA), dimethyl sulfoxide (DMSO), N,N-dimethylformamide (DMF), β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), xanthine, xanthine oxidase (XO), 9,9′-Bis(N-methylacridinium nitrate) (lucigenin), superoxide dismutase (SOD), catalase (CAT) thioredoxin (Trx), l-ascrobic acid, retinol, l-glutathione reduced (GSH), l-glutathione oxidized (GSSG), benzaldehyde-d₆ and Tween 80 were acquired from Sigma-Aldrich (Steinheim, Germany); pentobarbital sodium was purchased from Biowet (Puławy, Poland); chloro-2,4-dinitro benzene (CDNB), butylated hydroxytoluene (BHT), and 5,5′-dithiobis (2-dinitrobenzoic acid) (DTNB) were acquired from Sigma-Aldrich (Steinheim, Germany). DOCA was dissolved in DMF, whereas URB597 was dissolved in an URB597 solvent: a mixture of DMSO, Tween 80, and saline (0.9% NaCl) [1:2:7; v/v/v].