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Bradykinin

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Bradykinin is a nonapeptide involved in various physiological processes. It is utilized as a laboratory reagent for research purposes.

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Lab products found in correlation

Tropisetron hydrochloride, by Merck Group (2 mentions) Thapsigargin, by Bio-Techne (2 mentions) Ritanserin, by Merck Group (2 mentions) 6 1 1 dimethylethyl 2 2 furanylcarbonyl amino 4 5 6 7 tetrahydrobenzo b thiophene 3 carboxylic acid, by Bio-Techne (2 mentions) Xe991 10 10 bis 4 pyridinylmethyl 9 10h anthracenone, by Bio-Techne (2 mentions) 4 4 fluorobenzoyl 1 4phenylbutyl piperidine 4 f 4 pp oxalate, by Santa Cruz Biotechnology (2 mentions) Sb204741, by Bio-Techne (2 mentions) Phalloidin tritc, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions) Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions) Sodium hydroxide (naoh), by Avantor (2 mentions) Tween 20, by Merck Group (2 mentions) D glucose, by Thermo Fisher Scientific (2 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions) Pyruvate, by Merck Group (2 mentions) Pbs sachets, by Merck Group (2 mentions) Af 633 a30634, by Thermo Fisher Scientific (2 mentions) Cacl2 2h2o, by Merck Group (2 mentions) Mgso4 7h2o, by Avantor (2 mentions)

Close spelling variants of this product

[Phe8ψ(CH-NH)-Arg9]-bradykinin (3 citations) Bradykinin (BK) (2 citations)

8 protocols using bradykinin

1

Neurochemical Reagents for Receptor Studies

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5-Hydroxytryptamine (serotonin, 5HT), gamma-aminobutyric acid (GABA), capsaicin, ritanserin, tropisetron hydrochloride along with bulk chemicals were obtained from Sigma-Aldrich (Vienna, Austria). Bradykinin, 6-(1,1-Dimethylethyl)-2-[(2-furanylcarbonyl) amino]-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (CaCC Inh. - A01), SB 204741 (N-(1-Methyl-1H-5-indolyl)-N′ -(3-methyl-5-isothiazolyl)urea), thapsigargin, and XE991 (10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone) were purchased from Tocris (Bristol, UK). 4-(4-fluorobenzoyl)-1-(4phenylbutyl) piperidine (4 F 4 PP) oxalate was obtained from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany), capsazepine from Sanova Pharma Ges. m. b. H. (Vienna, Austria), 8-[5-(2,4-dimethoxy-5-(4-trifluoromethylphenylsulphonamido) phenyl-5-oxopentyl]-1,3,8-triazaspiro[4,5]decane-2,4-dione (RS102221) from Szabo-Scandic (Vienna, Austria), and tetrodotoxin (TTX) from Latoxan (Valence, France).
Salzer I., Gantumur E., Yousuf A, & Boehm S. (2016). Control of sensory neuron excitability by serotonin involves 5HT2C receptors and Ca2+-activated chloride channels. Neuropharmacology, 110(Pt A), 277-286.
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2

Opioid and Receptor Modulator Assays

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Naloxone (NLX), nordihydroguaiaretic acid (NDGA) and paroxetine were from Cayman Chemical. GFX 109203X (GFX) and CTAP were from Tocris Bioscience. [D-Ala2, N-Me-Phe4, Gly5-ol]-Enkephalin (DAMGO), bradykinin and common reagents were from Sigma-Aldrich.
Zhang Y, & Jeske N.A. (2020). GRK2 Dictates A Functional Switch of Peripheral Mu-Opioid Receptor. ACS chemical neuroscience, 11(24), 4376-4386.
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3

Neurochemical Reagents for Receptor Studies

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5-Hydroxytryptamine (serotonin, 5HT), gamma-aminobutyric acid (GABA), capsaicin, ritanserin, tropisetron hydrochloride along with bulk chemicals were obtained from Sigma-Aldrich (Vienna, Austria). Bradykinin, 6-(1,1-Dimethylethyl)-2-[(2-furanylcarbonyl) amino]-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (CaCC Inh. - A01), SB 204741 (N-(1-Methyl-1H-5-indolyl)-N′ -(3-methyl-5-isothiazolyl)urea), thapsigargin, and XE991 (10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone) were purchased from Tocris (Bristol, UK). 4-(4-fluorobenzoyl)-1-(4phenylbutyl) piperidine (4 F 4 PP) oxalate was obtained from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany), capsazepine from Sanova Pharma Ges. m. b. H. (Vienna, Austria), 8-[5-(2,4-dimethoxy-5-(4-trifluoromethylphenylsulphonamido) phenyl-5-oxopentyl]-1,3,8-triazaspiro[4,5]decane-2,4-dione (RS102221) from Szabo-Scandic (Vienna, Austria), and tetrodotoxin (TTX) from Latoxan (Valence, France).
Salzer I., Gantumur E., Yousuf A, & Boehm S. (2016). Control of sensory neuron excitability by serotonin involves 5HT2C receptors and Ca2+-activated chloride channels. Neuropharmacology, 110(Pt A), 277-286.
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4

Comprehensive Chemical Reagents for Cell Experiments

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NaCl, KCl, and d‐glucose were purchased from Fisher Scientific (Loughborough, UK); MOPS, EDTA, CaCl2·2H2O, pyruvate, PBS sachets, Tween20, and bovine serum albumin were purchased from Sigma (Poole, UK); and MgSO4·7H2O, NaOH, and NaH2PO4·H2O were purchased from VWR (Leicestershire, UK). Bradykinin and U46619 were purchased from Tocris (Bristol, UK). The structural dyes Hoechst (H3570), PI (P1304MP), DAPI (D3571), and AF‐633 (A30634) were purchased from ThermoFisher Scientific (Paisley, UK) and phalloidin‐TRITC from Sigma‐Aldrich (Dorset, UK). Paraformaldehyde was purchased from Electron Microscope Sciences (Hatfield, PA).
Dora K.A., Stanley C.P., Al Jaaly E., Fiorentino F., Ascione R., Reeves B.C, & Angelini G.D. (2016). Isolated Human Pulmonary Artery Structure and Function Pre‐ and Post‐Cardiopulmonary Bypass Surgery. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(2), e002822.
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5

Angiogenic Effects of Bradykinin

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A total of 60 μl Matrigel (10 mg/ml, Corning Inc.) was coated in the wells of a 96-well plate, and 104 HUVECs in 100 μl medium were then placed onto the layer of Matrigel in each well. The concentration variable (0.1, 1 and 10 μM) Bradykinin (Tocris Bioscience) and the Bradykinin B2 receptor antagonist, HOE140, (Tocris Bioscience) were supplemented to the corresponding wells. Medium alone served as the control. Tube morphogenesis was assessed under an Olympus IX73 microscope (Olympus) within 12 h. Each group had triplicate wells and the total tube length was quantified using ImageJ 1.8.0 software.
Zhou Y., Wang W., Wei R., Jiang G., Li F., Chen X., Wang X., Long S., Ma D, & Xi L. (2019). Serum bradykinin levels as a diagnostic marker in cervical cancer with a potential mechanism to promote VEGF expression via BDKRB2. International Journal of Oncology, 55(1), 131-141.
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6

Calcium Signaling in Pancreatic Stellate Cells

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Animals: C57BL6/J mice (Charles Rivers). Antibodies: Alexa Fluor 488 goat anti-mouse, Alexa Fluor 635 goat anti-rabbit (Thermo Fisher Scientific); mouse anti-BDKRB2 (Santa Cruz); rabbit anti-NOS2 (Merck). Cell culture: human pancreatic stellate cell line, SteCM complete stellate cell medium (ScienCell). Chemicals: aminoguanidine (AG), bradykinin, l-NAME (Tocris); PBS, ProLong Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific); other chemicals were obtained from Sigma. Fluorescent dyes: DAF-2 (Santa Cruz); DAF-FM, Fluo-4, Fura-2, Hoechst 33342, propidium iodide (Thermo Fisher Scientific).
Jakubowska M.A., Ferdek P.E., Gerasimenko O.V., Gerasimenko J.V, & Petersen O.H. (2016). Nitric oxide signals are interlinked with calcium signals in normal pancreatic stellate cells upon oxidative stress and inflammation. Open Biology, 6(8), 160149.
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7

Pharmacological Agents for Cell Studies

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The drugs apamin, charybdotoxin, tetraethylammonium chloride, atropine, haloperidol, pindolol, yohimbine, prazosin, phenylpherine, clonidine, caffeine, glibenclamide, β-funaltrexamine, naltrindole, nor-binaltorphimine, all purchased from Sigma Aldrich (U.S.A.) and bradykinin (Tocris Bioscience, U.K.), were prepared at the desired dose by dissolving them in distilled water (dH2O). Phorbol 12-myristate 13-acetate (Sigma Aldrich, USA) was dissolved in PBS solution. Acetic acid and dimethyl sulfoxide (DMSO) were purchased from Fisher Scientific (U.K.).
Zakaria Z.A., Sani M.H., Cheema M.S., Kader A.A., Kek T.L, & Salleh M.Z. (2014). Antinociceptive activity of methanolic extract of Muntingia calabura leaves: further elucidation of the possible mechanisms. BMC Complementary and Alternative Medicine, 14, 63.
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8

Comprehensive Reagent Procurement for Cell-Based Assays

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NaCl, KCl, and D-glucose were purchased from Fisher Scientific (Loughborough, UK); MOPS, EDTA, CaCl2 2H2O, pyruvate, PBS sachets, Tween20, and bovine serum albumin were purchased from Sigma (Poole, UK); MgSO4 7H2O, NaOH, and NaH2PO4 H2O were purchased from VWR (Leicestershire, UK); and bradykinin was purchased from Tocris (Bristol, UK). The cellular dyes Hoechst 33342 (H3570), PI (P1304MP), DAPI (D3571), and AF-633 (A30634) were purchased from ThermoFisher Scientific (Paisley, UK), phalloidin-TRITC from Sigma-Aldrich (Dorset, UK), and fluo-8 AM from AAT Bioquest (Stratech, Newmarket, UK). Paraformaldehyde was purchased from Electron Microscope Sciences (Hatfield, PA, USA).
Dora K.A., Borysova L., Ye X., Powell C., Beleznai T.Z., Stanley C.P., Bruno V.D., Starborg T., Johnson E., Pielach A., Taggart M., Smart N, & Ascione R. (2021). Human coronary microvascular contractile dysfunction associates with viable synthetic smooth muscle cells. Cardiovascular Research, 118(8), 1978-1992.
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