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2 protocols using hypochlorous acid

1

Antioxidant Assay Reagent Procurement

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Nicotine free base, hypochlorous acid, reduced L-glutathione, vitamin C (L-ascorbic acid), vitamin E analogue (Trolox: (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), and 3,3`,5,5`-tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich (St Louis, MO, USA). N-acetyl-L-cysteine was purchased from Calbiochem (Darmstadt, Germany). Glass microfiber filters (GF/C type, 2.4 cm diameter made by Whatman, UK) was purchased from Fisher Scientific (Fair Lawn, N.J). Guanidine hydrochloride (molecular biology grade and DNAse free) was purchased from Promega Corporation (Madison, WI). Tritiated thymidine was purchased from Amersham Biosciences (Piscataway, NJ).
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2

Cytotoxicity Assay of Chemical Compounds

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4-Cyanobenzaldehyde, benzil, 4-bromobenzoic acid, bis(pinacolato)diboron, 1,1′-carbonyldiimidazole (CDI), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II)dichloride (PdCl2 (dppf)), H2O2, potassium super-oxide (KO2), t-butyl hydroperoxide (TBHP), m-chloroperoxybenzoic acid (mCPBA), hypochlorous acid (HOCl), HEPES buffer solution, Whatman papers, Dulbecco's phosphate-buffered saline (DBS), Roswell Park Memorial Institute (RPMI) medium, fetal bovine serum (FBS), penicillin, streptomycin, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium acetate, acetic acid, ethanol, hydroxylamine hydrochloride, sodium hydroxide, ethyl acetate, hexane, sodium bicarbonate, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,4-dioxane, and potassium acetate were purchased from Daejung Chemicals (Siheungsi, Kyunggido, Republic of Korea). Sodium nitrate (NaNO3), potassium perchlorate (KClO4), sodium sulfate (Na2SO4), sodium nitrite (NaNO2), and benzoyl peroxide (BPO) were obtained from AccuStandard (New Haven, CT, USA). The MCF-7 cells were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea). All reagents were used as received without further purification.
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