Genemapper id

MDA-MB-231 (basal, ER-, PR-, HER2-), MDA-MB-453 (luminal, ER-, PR-, HER2-low), MDA-MB-468 (basal, ER-, PR-, HER2-), MCF-7 (luminal, ER+, PR+, HER2-), BT-474 (luminal, ER-, PR-, HER2+) and SK-BR-3 (luminal, ER-, PR-, HER2+) mammary carcinoma cell lines were obtained from ATCC (LGC Standards) and grown in Dulbecco modified Eagle medium (DMEM, Lonza) supplemented with 10% fetal bovine serum (FBS, SIGMA).
STAT3-silenced mammary carcinoma cells were generated by lentiviral transduction of pLKO vectors encoding for human shRNAs of the MISSiON system (sh2_TRCN0000020842, sh3_TRCN0000020843, SIGMA).
All cell lines were authenticated by BMR Genomics srl Padova, Italia, on January 2012 according to Cell ID ^™ System (Promega) protocol and using Genemapper ID Ver 3.2.1, to identify DNA STR profiles.

Ovarian cancer cell lines A2780 and A2780cis were from Sigma-Aldrich; SKOV3 (HTB-77) and COV318 were obtained from the American Type Culture Collection (ATCC); OVCAR5 (NIH) cells were provided by Dr. Baldassarre (CRO, Aviano, Italy); Kuramochi cells (JCRB0098), resistant to paclitaxel [15 ], were from JCRB Cell Bank; and OVCAR8 cells were from the National Cancer Institute Developmental Therapeutics Program Tumor Repository. All cell lines were routinely tested for mycoplasma, with negative results, and authenticated in our laboratory using PowerPlex 16 HS System (Promega, Madison, WI, USA) and GeneMapper ID version 3.2.1 to identify DNA short tandem repeats. ADSCs (Lonza) were maintained in Mesenchymal-Stem-Cell Growth Medium Bulletkit MSCGM (Lonza, Verviers, Belgium). Ovarian cancer cell lines were maintained in RPMI-1640 medium (Sigma-Aldrich Co., St. Louis, MO, USA) containing 10% heat-inactivated FBS, 100 U/mL penicillin and streptomycin (complete medium), at 37 °C in a 5% CO2 atmosphere. A2780cis cells were maintained in 1 µM cisplatin and cultured without the drug for 72 h before use in cellular assays. To obtain 3D-multicellular tumor spheroids (MCTSs), plates were coated twice with 20 mg/mL of poly(2-hydroxyethyl methacrylate) (poly-HEMA; Sigma) in 95% ethanol and washed once with PBS before cell seeding (2.0 × 10⁴ cells in 24-well).

Primary wild type (WT) and p27 knock-out (p27KO) MEF and 3T3 cells were prepared and cultured as previously described [36 (link)]. 293T/17 were used for the production of retroviral particles and cultured in DMEM supplemented with 10% FBS (Sigma). Normal breast and tumor derived cells were cultured as described in SI. All utilized cell lines were authenticated by BMR Genomics srl, Padova, Italia, on January 2012 according to Cell ID™ System (Promega) protocol and using Genemapper ID Ver 3.2.1 to identify DNA STR profiles.