Polyclonal anti src py418 phospho specific antibody

Manufactured by Thermo Fisher Scientific

The Polyclonal anti-Src [pY418] phospho-specific antibody is a laboratory tool used to detect and quantify the phosphorylation of the tyrosine 418 residue in the Src protein. This antibody is designed for use in various analytical techniques, such as Western blotting, immunoprecipitation, and immunofluorescence.

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Lab products found in correlation

Antibodies against phospho erk1 2 and total erk1 2, by Cell Signaling Technology (1 mentions) Polyclonal antibody, by Cell Signaling Technology (1 mentions) 86rbcl, by PerkinElmer (1 mentions) 2 4 dinitrophenylhydrazine, by Merck Group (1 mentions) 22nacl, by PerkinElmer (1 mentions)

2 protocols using polyclonal anti src py418 phospho specific antibody

Quantifying Cardiac Tissue Oxidation

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Cardiac (left ventricle, LV) tissue samples and cardiac fibroblast homogenates were prepared and analyzed as we previously described for collagen-125 (link) and other proteins5 (link). Assessment of protein carbonylation and activation of c-Src and ERK1/2 was performed as we described in ref. 5 (link). Multiple exposures were analyzed to assure that the signals were within the linear range of the film. The signal density was determined using NIH ImageJ 1.48 v software. Polyclonal antibody against type I collagen was from Southern Biotech (Birmingham, AL). Polyclonal anti-Src [pY418] phospho-specific antibody was from Invitrogen (Camarillo, CA). Monoclonal antibody against total c-Src was from Santa Cruz (Santa Cruz, CA). Antibodies against phospho-ERK1/2 and total ERK1/2 were obtained from Cell Signaling Technology (Danvers, MA). 2,4-dinitrophenylhydrazine (DNPH) and antibody against 2,4-dinitrophenyl (DNP) hydrazone derivatives were from Sigma-Aldrich.

Liu J., Tian J., Chaudhry M., Maxwell K., Yan Y., Wang X., Shah P.T., Khawaja A.A., Martin R., Robinette T.J., El-Hamdani A., Dodrill M.W., Sodhi K., Drummond C.A., Haller S.T., Kennedy D.J., Abraham N.G., Xie Z, & Shapiro J.I. (2016). Attenuation of Na/K-ATPase Mediated Oxidant Amplification with pNaKtide Ameliorates Experimental Uremic Cardiomyopathy. Scientific Reports, 6, 34592.

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Analyzing Na/K-ATPase Regulation and Signaling

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All chemicals, except otherwise mentioned, were obtained from Sigma‐Aldrich (St. Louis, MO). Monoclonal antibodies against Na/K‐ATPase α1 subunit (clone α6F and clone C464.6) and β1 (clone C464.8) were from the Developmental Studies Hybridoma Bank at the University of Iowa (Iowa City, IA) and EMD Millipore Upstate (Billerica, MA), respectively. Polyclonal anti‐rat α1‐specific antibody (anti‐NASE) was kindly provided by Dr Pressley (Texas Tech University Health Sciences Center, Lubbock, TX). Polyclonal antibody against early endosome marker Rab5 was from Cell Signaling Technology (Danvers, MA). Polyclonal anti‐Src (pY418) phosphospecific antibody was from Invitrogen (Camarillo, CA). Monoclonal antibodies against total c‐Src and tyrosine phosphorylation (p‐Tyr, clone PY99) were from Santa Cruz (Santa Cruz, CA). 2,4‐Dinitrophenylhydrazine and antibody against 2,4‐dinitrophenyl hydrazone derivatives were from Sigma‐Aldrich. Radioactive [³H]‐ouabain, ⁸⁶RbCl, and ²²NaCl were from Perkin Elmer (Shelton, CA).

Yan Y., Shapiro A.P., Mopidevi B.R., Chaudhry M.A., Maxwell K., Haller S.T., Drummond C.A., Kennedy D.J., Tian J., Malhotra D., Xie Z., Shapiro J.I, & Liu J. (2016). Protein Carbonylation of an Amino Acid Residue of the Na/K‐ATPase α1 Subunit Determines Na/K‐ATPase Signaling and Sodium Transport in Renal Proximal Tubular Cells. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(9), e003675.

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