Uw 2070 probe

Keto-HPC with a DOx of 1.5 was dissolved in Mili-Q^® water to result in a 4 wt% solution. A total of 25 g of a fresh keto-HPC solution was added to a 50 mL round glass. For ultrasonic treatment, a BANDELIN electronic UW 2070 probe was immersed into the keto-HPC solution for 1.5 cm. Before the ultrasonic treatment, the keto-HPC solution was cooled down with an ice bath for 15 min while stirring with 700 RPM. The cold solution was treated with ultrasound for 0.5 h, 1.0 h, 2 h, 4 h and 8 h, respectively, using the BANDELIN electronic UW 2070 in combination with a BANDELIN Sonoplus with a pulse of 7 and power of 70 ± 1%. The degraded keto-HPC was dried via lyophilization for several days and stored in the fridge at −18 °C for further analysis and application.

Solid lipid nanoparticles were prepared by an ultrasonic-assisted hot oil in water emulsion method [45 (link),46 (link)]. The lipid phase composed of Stearic Acid and soy PC was melted under magnetic stirring and kept 10 °C above the Stearic Acid melting point, which is 69.3 °C. A volume of 500 µL of a standard solution of Triamcinolone Acetonide (1 mg/mL in ethanol) was added in the lipid phase. Separately, the aqueous phase containing the non-ionic surfactant (Tween 80) was brought to the same temperature and slowly added to the melted lipid phase. The O/W mixture was kept under magnetic stirring (400 rpm) and at 79 °C for 30 min to create a pre-emulsion and to let the ethanol evaporate, and it was then sonicated in an ice bath for 10 min, with 50% on/off cycles using a probe sonicator (Bandelin Sonoplus HD2070 equipped with a UW 2070 probe, BANDELIN electronic, Berlin, Germany). The resulting solid lipid nanoparticle dispersions were stored at 4 °C until further characterization. All of the tests on the prepared formulation were measured on the same day as the synthesis.