Oci ly19

Manufactured by Thermo Fisher Scientific

Sourced in Switzerland, France

The OCI-LY19 is a laboratory instrument designed for performing optical characterization and imaging of materials. It provides high-resolution imaging and analysis capabilities for a variety of samples.

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Lab products found in correlation

5 protocols using oci ly19

Establishment and Characterization of DLBCL Cell Lines

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Non-GCB type DLBCL cell-line NU-DUL and GCB type DLBCL cell-lines OCI Ly1 and OCI Ly18 were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Germany). Non-GCB type DLBCL cell-lines OCI Ly3, OCI Ly10 and GCB type DLBCL cell-line OCI Ly19 were a kind gift of Dr. F. Bertoni, Bellinzona, Switzerland. NU-DUL were maintained in RPMI 1640 medium supplemented with 15% fetal bovine serum (FCS) + 2-Mercaptoethanol (50mM; Invitrogen); OCI Ly10, OCI Ly3 and OCI Ly1 in IMDM 20% FCS + 2-Mercaptoethanol (50mM); OCI Ly18 in RPMI 10% FCS + 2-Mercaptoethanol (50mM) and OCI Ly19 in RPMI 10% + 2-Mercaptoethanol (50mM) + MEM NEAA + sodium pyruvate. BL Raji cell-line was purchased from ATCC and cultured in RPMI 20% FCS. Testing for Mycoplasma infection was carried at a monthly basis. Peripheral blood mononuclear cells (PBMCs) and B lymphocytes from healthy donors were obtained from peripheral blood as per standard Ficoll Paque^® protocol; B lymphocytes were purified using CD19-coated magnetic MicroBeads according to the manufacturer's protocol (Miltenyi Biotech).

Pizzi M., Piazza F., Agostinelli C., Fuligni F., Benvenuti P., Mandato E., Casellato A., Rugge M., Semenzato G, & Pileri S.A. (2015). Protein kinase CK2 is widely expressed in follicular, Burkitt and diffuse large B-cell lymphomas and propels malignant B-cell growth. Oncotarget, 6(9), 6544-6552.

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Culturing 16 DLBCL Cell Lines

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The 16 DLBCL cell lines (DOHH2, HT, OCI-LY19, DB, OCI-LY1, SUDHL-4, SUDHL-5, SUDHL-10, NUDHL-1, OCI-LY7, WSU-DLCL2, SUDHL-6, NUDUL-1, U2932, OCI-LY3, and RI-1) were purchased from the American Type Culture Collection or from DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany). They were grown in RPMI-1640 Glutamax medium (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 10% fetal bovine serum (FBS) (PAA laboratory GmbH, Pasching, Austria) (U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, and WSU-DLCL2 cells), 20% FBS (OCI-LY3, DB, SUDHL-5, NUDHL-1, and SUDHL-6 cells), or 15% FBS (NUDUL-1 cells). OCI-LY1, and OCI-LY7 cells were cultured in IMDM Glutamax (Gibco, Invitrogen, Cergy Pontoise, France), supplemented with 20% FBS, and OCI-LY19 cells in MEM alpha modified Glutamax (Gibco, Invitrogen, Cergy Pontoise, France) with 20% FBS. Cultures were maintained at 37 °C in a humidified atmosphere with 5% CO₂. Contamination by Mycoplasma species was regularly monitored.

Devin J., Kassambara A., Bruyer A., Moreaux J, & Bret C. (2019). Phenotypic Characterization of Diffuse Large B-Cell Lymphoma Cells and Prognostic Impact. Journal of Clinical Medicine, 8(7), 1074.

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DLBCL Cell Line Maintenance Protocol

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The 16 DLBCL cell lines (U2932, OCI-LY-3, NU-DHL-1, OCI-LY-19, DB, SUDHL4, OCILY1, SUDHL5, DOHH2, SUDHL10, HT, RI-1, SU-DHL-6, NUDUL-1, WSU-DLCL-2 and OCI-LY-7) were purchased from the DSMZ (Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH). They were maintained in RPMI-1640 (Gibco, Invitrogen), supplemented with 10% FBS (PAA laboratory GmbH) for U2932, SUDHL-4, HT, DOHH2, SUDHL-10, RI-1, WSU-DLCL-2 cell lines, 20% FBS OCI-LY3, DB, SUDHL-5, NUDHL-1, SU-DHL-6, NUDUL-1 cell lines. OCI-LY1 and OCI-LY7 were cultured in IMDM (Gibco, Invitrogen), supplemented with 20% FBS and OCI-LY19 was cultured in MEM alpha modified (Gibco, Invitrogen), supplemented with 20% FBS. Cell line identity was regularly checked by short-tandem repeat analysis, and cells were regularly screened for Mycoplasma contamination.

Devin J., Cañeque T., Lin Y.L., Mondoulet L., Veyrune J.L., Abouladze M., Garcia De Paco E., Karmous Gadacha O., Cartron G., Pasero P., Bret C., Rodriguez R, & Moreaux J. (2022). Targeting Cellular Iron Homeostasis with Ironomycin in Diffuse Large B-cell Lymphoma. Cancer Research, 82(6), 998-1012.

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Cell Culture Protocols for Leukemia and Lymphoma

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Four AML cell lines, OCI-AML3, HEL, KG-1 and HL-60, and two diffuse large B-cell lymphoma (DLBCL) cell lines, OCI-LY1 and OCI-LY19, were purchased from DSMZ (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures). One DLBCL cell line, TOLEDO, was purchased from the American Type Culture Collection (ATCC). HEL, KG-1 and HL-60 cells were cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum (FBS), l-glutamine and 1% penicillin–streptomycin (Gibco, Grand Island, NY, USA) at 37 °C and 5% CO₂. OCI-AML3 and OCI-LY19 cells were cultured in α-MEM supplemented with 20% FBS, l-glutamine and 1% penicillin–streptomycin (Gibco) at 37 °C in the presence of 5% CO₂. OCI-LY1 cells were cultured in Iscove Modified Dulbecco Media (IMDM) supplemented with 20% FBS, l-glutamine and 1% penicillin–streptomycin (Gibco, Grand Island, NY, USA) at 37 °C and 5% CO₂.

Liu J., Hong J., Han H., Park J., Kim D., Park H., Ko M., Koh Y., Shin D.Y, & Yoon S.S. (2020). Decreased vitamin C uptake mediated by SLC2A3 promotes leukaemia progression and impedes TET2 restoration. British Journal of Cancer, 122(10), 1445-1452.

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Culturing Diverse B-Cell Lymphoma Lines

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Human normal B-cell immortalized cell line (HMy2.CIR), DLBCL cell line, germinal central B-cell (GCB)-like OCI-Ly19 and SU-DHL-4, and ABC-like OCI-LY10 and U2932 were purchased from Shanghai Cell Bank of the Chinese Academy of Sciences. HMy2.CIR was cultured in Iscove’s modified dulbecco’s medium (IMDM) (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), and 1% penicillin–streptomycin (P/S). U2932 and SU-DHL-4 were cultured in RPMI 1640 medium (Gibco) containing 10% FBS and 1% P/S. OCI-LY10 and OCI-Ly19 were cultured in IMDM (Gibco) containing 20% FBS and 1% P/S. All cells were maintained in a humid incubator with 5% CO₂ at 37°C.

Sun S., Wang H, & Ji M. (2019). Overexpression of miR-222-3p Promotes the Proliferation and Inhibits the Apoptosis of Diffuse Large B-Cell Lymphoma Cells via Suppressing PPP2R2A. Technology in Cancer Research & Treatment, 18, 1533033819892256.

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