Dibasic sodium phosphate

Chitosan quaternary ammonium salt (HACC) with an 95% degree of quaternization, and budesonide (BUD, 98%) were purchased from Macklin (Shanghai, China). Sodium cellulose sulfate (NaCS) was provided by Institute of Bioengineering, Zhejiang University. Cellulase was ordered from Meilunbio (Dalian, China). Lauroyl chloride, pyridine, and iron oleate were obtained from Aladdin (Shanghai, China). Compritol® 888 ATO was ordered from Gattefosse (Shanghai, China). Dextran sodium sulfate (DSS) was obtained from Yeasen (Shanghai, China). Pyrene was provided by Shanghai Dibo Chemicals Technology Co., Ltd. (Shanghai, China). Ethanol, acetaldehyde, sodium chloride, potassium chloride, dibasic sodium phosphate, and potassium dihydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). 3, 5-Dinitrosalicylic acid (DNS) reagent was obtained from Phygene Biotechnology Co., Ltd (Fuzhou, China).

PhosphatidylSerine (PS) was purchased from Chemi Nutra Inc. (Austin, TX, USA). Phosphatidylcholine (PC) was purchased from Shanghai Macklin Biochemical Co., Ltd. Curcumin, citric acid, α-D-Lactose monohydrate, trehalose, and mannitol were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Sodium dihydrogen phosphate and dibasic sodium phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Artificial saliva (CZ0243, pH6.8), simulated gastric fluid (CZ0212), and artificial intestinal juice (CZ0200) were purchased from Beijing Leagene Biotechnology Co., Ltd. (Beijing, China). Cell Counting Kit-8 (CCK-8), Dulbecco’s modified Eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). Hydrogen peroxide (H₂O₂) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Reactive oxygen species (ROS) assay kit was purchased from Beijing Applygen Technology Co., Ltd. (Beijing, China). Ultra-pure water was obtained by MILLI-Q* ultra-pure water system.

The collagenase was supplied by Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). The elastase was obtained from Beijing Coolaber Technology Co., Ltd. (Beijing, China). The hyaluronidase from bovine testes and the fluorogenic MMP 2 substate (N-Succinyl-Ala-Ala-Ala-p-nitroanilide) were bought from Sigma-Aldrich (St Louis, MO, United States). The 10 kDa cutoff centrifugal ultrafiltration filters (YM-10) were purchased from Millipore Co., Ltd. (Bedford, MA, United States). The membranes (0.22 μm) were bought from Tianjin Jinteng Experiment Equipment Co., Ltd. (Tianjin, China). Epigallocatechin gallate was bought from Shanghai Meryer Chemical Technology Co., Ltd. (Shanghai, China). Acetonitrile (ACN) and formic acid (FA) of HPLC grade were purchased from TEDIA Company Inc. (Fairfield, Ohio, USA). The ultrapure water for LC-MS analysis and pure water for the sample process were prepared using the EPED water system (Nanjing EPED Technology Development Co., Ltd, Nanjing, China). All other chemicals were of analytical grade and supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), including methanol (MeOH), hydrochloric acid (HCl), sodium dihydrogen phosphate (NaH₂PO₄), dibasic sodium phosphate (Na₂HPO₄), and ascorbic acid.

Cucurbit[7]uril (CB[7]) was synthesized following a procedure published by Day's group (Day et al., 2001 (link)). Gold wire, sodium chloride, sodium dihydrogen phosphate, dibasic sodium phosphate, sodium hydroxide, hydrochloric acid and absolute ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd. Melphalan was purchased from aladdin. 1, 2, 4-trichlorobenzene was purchased from Sigma-Aldrich. All the purchased chemicals were used directly without further purification. Deionized water from a Milli-Q water purifying system was used to prepare all of the solutions (18.2 MΩ).

Cyromazine was purchased from Zhengzhou Labor Agrochemicals Co., Ltd., Zhengzhou, China); concentrated hydrochloric acid, sodium dihydrogen phosphate, dibasic sodium phosphate, glutaric dialdehyde, and potassium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China); potassium hexacyanoferrate(II) was purchased from Xilong Scientific Co., Ltd. (Guangzhou, China); potassium ferricyanide was purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China); α-methacrylic acid was purchased from Meryer (Shanghai, China) Chemical Technology Co., Ltd. (Shanghai, China); trimethylolpropane trimethacrylate was purchased from Hubei Shishun Bio-Technology Co., Ltd. (Huanggang, China); acetonitrile was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). All the above reagents were analytically pure. Cowpea and tomato were purchased at a nearby farmers’ market. Chloroauric acid (99%) was purchased from Hubei Guangao Biotechnology Co., Ltd. (Wuhan, China). Water samples were collected from paddy fields (19.507036° N, 109.504222° E), rivers (19.513888° N, 109.4917.2° E), and botanical gardens (19.5124° N, 109.4988° E).

Potassium chloride (KCl), sodium chloride (NaCl), sodium sulphate (Na₂SO₄), dibasic sodium phosphate (Na₂HPO₄), potassium dihydrogen phosphate (KH₂PO₄), and Uric acid (UA) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dopamine (DA) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All of the above chemical reagents were analytical reagents and were used without further purification. GO powder and rGO water dispersion were purchased from Nanjing JCNANO Technology Co., Ltd. (Nanjing, China). Ti₃C₂T_x water dispersion was purchased from Beike 2D materials Co., Ltd. (Suzhou, China). Deionized Milli-Q water (18.2 MΩ/cm) was used throughout the experiments.