Tera 1

The human teratocarcinoma cell line Tera-1 (ATCC, Manassas, VA, USA, Cat# HTB-105) was grown in McCoy’s 5A media (Life Technologies, Carlsbad, CA, USA, Cat# 16600-082), supplemented with 15% FBS (Atlanta Biologicals, Norcross, GA, USA, Cat# S11195) and 1% Pen-Strep (Life Technologies Cat# 15140-122). Feline astrocyte G355.5 cells (ATCC Cat# CRL-2033) were grown in McCoy’s 5A media supplemented with 10% FBS and 1% Pen-Strep. 293T cells (ATCC Cat# CRL-2316) were grown in DMEM supplemented with 10% FBS and 1% Pen-Strep. All cell lines were grown at 37 °C with 5% CO₂.

HEK-293T, NCI-H1299, BxPC-3, PANC-1, Hep3B, PLC/PRF/5,HepG2, SK-HEP-1, MCF7, A549, NCI-H460, Tera-1 and Tera-2 were purchased from ATCC; HuH-7 was purchased from Japanese Collection of Research Bioresources (JCRB), SMMC-7721 and BEL-7402 were purchased from Typical culture preservation commission cell bank, Chinese academy of sciences (NCB); MHCC-97L and LM3 were gifts from Zhongshan Hospital, Fudan University (Shanghai, China); SMMC-7721, BEL-7402, MHCC-97L and LM3 used in this study have been described in previous publication [24] (link), [30] (link), [31] , [32] (link).
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO₂.

HEK293T, TERA-1 and NT2/D1 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA). Anti-HIF-1α antibody was purchased from Bethyl Laboratories (Montgomery, TX). Antibodies to α-tubilin, PARP, and HIF-2α were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against GFP, NANOG, and OCT4 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). SALL4 antibody was purchased from Abcam (Cambridge, MA). Human embryonic stem cells (H1, WiCell, WI) were cultured using a feeder-dependent culture condition. These cells were maintained in DMEM-F12 (Invitrogen, USA) medium which was supplemented with 20% KSR, 10 ng/mL bFGF, 2mM GlutaMAX™-I, 0.1 mM MEM Non-Essential Amino Acids Solution, 1×β-mercaptoethanol. Cells were passed every other day after trypsinization. Mitomycin C treated murine embryonic fibroblasts (MEFs) were prepared as feeder cells.