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Basic fibroblast growth factor (bfgf)

Manufactured by RayBiotech
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The BFGF product is a reagent used in research applications. It serves as a basic fibroblast growth factor, which is a protein involved in cellular processes. This product provides a source of this growth factor for experimental use.

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Lab products found in correlation

Vegf a, by RayBiotech (2 mentions) Pdgf bb, by RayBiotech (2 mentions) Elisa plate reader, by Agilent Technologies (1 mentions) Vascular endothelial growth factor (vegf), by RayBiotech (1 mentions) Vcam 1, by RayBiotech (1 mentions) Sicam 1, by RayBiotech (1 mentions) Hb egf, by RayBiotech (1 mentions) Leptin, by RayBiotech (1 mentions) Hepatocyte growth factor (hgf), by RayBiotech (1 mentions) Angiogenin, by RayBiotech (1 mentions) Ang 2, by RayBiotech (1 mentions) Epidermal growth factor (egf), by RayBiotech (1 mentions) Tgf β1, by R&D Systems (1 mentions) Vegf c, by R&D Systems (1 mentions) Ang 1, by R&D Systems (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Pd98059, by Apexbio (1 mentions) Pd173074, by Apexbio (1 mentions) Timp 3, by RayBiotech (1 mentions)

7 protocols using basic fibroblast growth factor (bfgf)

1

Plasma Biomarker Quantification Protocol

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Venous blood was obtained at baseline (0 week visit) and at 8 weeks. Patient plasma was immediately collected and stored at −80°C until use. Parameters measured included: VEGF, tPA, sE- Selectin, bFGF, VCAM-1, and sICAM-1 in patient plasma by ELISA using commercially available Kits (bFGF, VCAM-1, and sICAM-1: RayBiotech Inc, Norcross, GA;. tPA: Abcam, Cambridge, MA; .sE-selectin and VEGF: R&D Systems Inc, Minneapolis, MN; PINP: MyBioSource, San Diego, CA). Proper dilutions were made to ensure that the concentration fell within the standard curve. Samples were all above the detection limit of the assay. Samples were run in duplicate. Anti-human primary antibodies were coated on a 96-well plate before the addition of samples and standards. Sandwich ELISA was completed by adding biotinylated anti-human antibodies, followed by streptavidine-HRP and substrate solution. The optical density of each well was measured using an ELISA plate reader (BioTeK, Winooski, VT).
Gladue H., Berrocal V., Harris R., Tsou P.S., Edhayan G., Ohara R, & Khanna D. (2016). A Randomized Controlled Trial of Acupressure for the Treatment of Raynaud's Phenomenon: The difficulty of conducting a trial in Raynaud's phenomenon. Journal of scleroderma and related disorders, 1(2), 226-233.
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2

Profiling Angiogenic Factors in EC/VSMC

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Saphenous vein-derived ECs and VSMCs were separately cultured on 6-well plates and treated with TGFβ1 (0, 1, and 10 ng/ml) for 24 h. The resulting conditioned medium was harvested and used to determine the relative levels of AGFs by ELISA (VEGF-C, Ang-1, TGF-β1; R&D Systems Ltd) or Quantibody multiplex array (Angiogenin, Ang-2, EGF, bFGF, HB-EGF, HGF, Leptin, PDGF-BB, PlGF, VEGF-A; Raybiotech, Norcross, United States) according to the manufacturers’ instructions. Each experiment was performed on three separate occasions.
Lu Q., Sun D., Shivhare S.B., Hou H., Bulmer J.N., Innes B.A., Hapangama D.K, & Lash G.E. (2021). Transforming Growth Factor (TGF) β and Endometrial Vascular Maturation. Frontiers in Cell and Developmental Biology, 9, 640065.
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3

Bioactive Factors Secreted by SVF Cells

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Fresh mSVF pellet was suspended in minimum essential medium (MEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin-streptomycin. Conditioned medium was collected from SVF cells and after two days of single culture without media change and was analyzed immediately by following ELISA kits: basic fibroblast growth factor (bFGF; RayBiotech, Peachtree Corners, GA, USA; #ELR-bFGF-1; detection range 2–500 pg/mL), vascular endothelial growth factor A (VEGF-A; RayBiotech; Peachtree Corners, GA, USA; #ELR-vEGF-1; detection range 2–200 pg/mL) and tumor growth factor beta (TGF-b; Invitrogen, Carlsbad, CA, USA; TGF beta-1 Rat ELISA Kit; detection range: 31.25–2000 pg/mL).
Kim B.S., Chen S.H., Vasella M., Guidi M., Gousopoulos E., Lindenblatt N, & Kao H.K. (2022). In Vivo Evaluation of Mechanically Processed Stromal Vascular Fraction in a Chamber Vascularized by an Arteriovenous Shunt. Pharmaceutics, 14(2), 417.
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4

Investigating Neuroprotective Agents in TBI

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Animals were randomly assigned to the following groups: the sham-operated (sham group, n=6), the IAH+TBI group (combined group, n=6), the combined+bFGF group (n=6), the combined+bFGF+PD173074 group (n=6), and the combined+bFGF+PD98059 group (n=6). Before blood was drawn, rats in the combined+bFGF group were treated with bFGF (10 μg/kg; intraperitoneal (IP) injection; RayBiotech, Peachtree Corners, GA, USA) for 2 min. The rats in the combined+bFGF+PD173074 group were treated with the FGFR1 antagonist, PD173074 (10 mg/kg; IP injection; APExBIO, Houston, TX, USA) 2 min before the administration of bFGF. The rats in the combined+bFGF+PD98059 group were administrated PD98059 (ERK antagonist; 20 mg/kg; IP injection; APExBIO) 2 min before bFGF administration.
Chen P., Tang H., Zhang Q., Xu L., Zhou W., Hu X., Deng Y, & Zhang L. (2020). Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e922009-1-e922009-12.
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5

Quantifying Growth Factors in PRP

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PTP-001 eluates and LR-PRP or LP-PRP samples (N = 10 individual donors for each) were assayed using ELISAs from R&D Systems (Minneapolis, MN) for basic fibroblast growth factor (bFGF) and interleukin-1 receptor antagonist (IL-1Ra), and from RayBiotech (Peachtree Corners, GA) for tissue inhibitor of metalloproteases-3 (TIMP-3).
Flannery C.R., Buddin K.E., Begum L., Nasert M.A., Catalfamo B., Semler E.J, & Fortier L.A. (2023). Composition and Bioactivity of a Placental Tissue Particulate (PTP-001) Indicate Greater Potential than Platelet-Rich Plasma for the Treatment of Osteoarthritis. Cartilage, 14(4), 467-472.
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6

Quantifying Wound Healing Factors

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Specific components relevant to wound healing were identified and concentrations of these components were measured using enzyme-linked immunosorbent assay (ELISA) in each sample type. PDGF-BB, PDGF-AA, TIMP-1, TIMP-2, TIMP-4, TGF-β1, TGF-α, bFGF, and EGF ELISA kits were obtained from RayBiotech, Inc (Norcross, GA). Hyaluronan (Hyaluronic acid [HA] ) and VEGF ELISA kits were obtained from R&D Systems (Bio-Techne Corporation, Minneapolis, MN). Lactoferrin ELISA kits were obtained from AssayPro, LLC (St. Charles, MO). Assays were performed according to each kit manufacturer's instructions. Component concentrations in the IL are reported. To compare component concentrations, dCHPM component concentrations were normalized to the component concentrations of fresh, unprocessed PM.
Roy A, & Griffiths S. (2020). Intermediate layer contribution in placental membrane allografts. Journal of tissue engineering and regenerative medicine, 14(8).
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7

SYBR‐Green qPCR for TGFβ1 and bFGF

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SYBR‐Green quantitative polymerase chain reaction (q‐PCR) kit (Jena Bioscience, Germany), TGFβ1 (eBioscience, USA), and bFGF (RayBiotech, USA). Chemicals and biochemical reagents are of analytical grade and were freshly prepared and used.
Hormozi M, & Baharvand P. (2019). Achillea biebersteinni Afan may inhibit scar formation: In vitro study. Molecular Genetics & Genomic Medicine, 7(5), e640.
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