Empiria studio 1

Manufactured by LI COR

The Empiria Studio 1.2 is a compact and versatile spectrophotometer designed for a wide range of applications in life science research laboratories. It features a high-resolution, color touchscreen display, and offers precise measurements of absorbance and transmittance across a broad wavelength range.

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Lab products found in correlation

Gapdh, by LI COR (1 mentions) Revert 700 total protein stain solution, by LI COR (1 mentions) Anti mouse, by LI COR (1 mentions) Anti rabbit, by LI COR (1 mentions) Intercept tbs blocking solution, by LI COR (1 mentions) Infusion cloning primer design tool, by Takara Bio (1 mentions) Prism software, by GraphPad (1 mentions)

2 protocols using empiria studio 1

Immunoblotting Assay for Signaling Proteins

Cited 1 time

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Whole-cell lysates and immunoblotting were performed as described elsewhere (19 (link), 20 (link)). Blots were probed with the following antibodies obtained from Santa Cruz or CST (Danvers, MA, USA): phospho-ERK: Cat# sc-7383, RRID AB_627545, 1:1000; ERK: Cat# sc-514302, RRID : AB_2571739, 1:1000; SHP2: Cat# 3397, RRID: AB_2174959, 1:1000; PDGFRβ: Cat# sc-374573, RRID: AB_10990921, 1:100; pan-RAS: Cat# sc-166691, RRID: AB_2154229, 1: 200; GAPDH: Cat# sc-47724, RRID: AB_627678, 1:2000; anti-rabbit: Cat# 926-32211, RRID: AB_621843; Cat# 926-926-68071, RRID: AB_10956166; anti-mouse: Cat# 926-32210, RRID: AB_621842, Cat# 926-680707, RRID: AB_10956588; all 1:10,000, LiCOR (Bad Homburg, Germany). Primary antibodies were diluted in Intercept/TBS blocking solution (LiCOR) supplemented with 0.2% Tween-20, secondary antibodies were diluted in TBS supplemented with 0.1% Tween-20. Total protein staining was performed using Revert 700 Total Protein Stain Solution according to the manufacturer’s protocol (LiCOR). Densitometry was performed using Empiria Studio 1.2 (LiCOR).

Kaehler M., Osteresch P., Künstner A., Vieth S.J., Esser D., Möller M., Busch H., Vater I., Spielmann M., Cascorbi I, & Nagel I. (2023). Clonal evolution in tyrosine kinase inhibitor-resistance: lessons from in vitro-models. Frontiers in Oncology, 13, 1200897.

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Comprehensive Molecular Analysis Protocol

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Primers were designed using the InFusion Cloning primer design tool (Takara Bio Europe SAS). Densitometry was performed using Empiria Studio 1.2 (LI-COR Biosciences). Spearman-Rank correlation was calculated using Cluster 3.0 (Stanford University, Stanford, CA, USA). Unless not otherwise described, statistical analysis was performed using one-way ANOVA with subsequent Dunnett's test, unpaired Student's or Welch's t-test and the GraphPad prism software (Version 8.0; GraphPad Software, Inc.). P<0.05 was considered to indicate a statistically significant difference.

Kaehler M., Litterst M., Kolarova J., Böhm R., Bruckmueller H., Ammerpohl O., Cascorbi I, & Nagel I. (2022). Genome-wide expression and methylation analyses reveal aberrant cell adhesion signaling in tyrosine kinase inhibitor-resistant CML cells. Oncology Reports, 48(2), 144.

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