Tae buffer
TAE buffer is a commonly used buffer solution for electrophoresis applications. It is primarily used to maintain the pH and ionic environment during the separation of nucleic acids, such as DNA and RNA, in agarose gel electrophoresis.
Lab products found in correlation
23 protocols using tae buffer
SDD-AGE for Aggregates and Fibrils Detection
RAPD-PCR based genetic analysis
PCR was performed using Prime thermal cycler (with heated lid, 100 ºC; Techne) programmed for 40 cycles of denaturation (30 sec at 94 ºC), annealing (60 sec at 38 ºC) and extension (2 min at 72 ºC), with a final 10 min extension step (72 ºC). The reaction products were separated via agarose gel electrophoresis (1.5% in 1x TAE buffer [Fisher, UK] containing ethidium bromide [0.5 ng/mL]), using 1 Kb DNA ladder (Promega) as a molecular size indicator. DNA fragment patterns were visualized under UV light (Genesnap, Syngene) and analysed by Gel Compar II software.
Molecular Typing of Klebsiella Virulence
HAdV-5 Infection in siRNA-Transfected HeLa Cells
Gel Electrophoresis Protocol for DNA Separation
400 μL SYBR Safe DNA Gel Stain, DNA loading dye, SDS solution
(6×), TAE buffer (Tris–acetate–EDTA) (50×),
TrackIt 100 bp DNA Ladder, and Ultra Low Range DNA Ladder were purchased
from Thermo Fisher Scientific. Agarose was purchased from Bio-Rad
Laboratories. NuPAGE 4–12%, Bis–Tris, 1.0 mm, and Mini
Protein Gel (12-well) were purchased from Invitrogen.
High-Molecular-Weight DNA Extraction
For all species, HMW DNA was exclusively observed in the DNA samples extracted from cells homogenized using the LN2 grinding method. The DNA extracted using this treatment was observed as a tight, clear band over the 21.2‐kbp marker band, whereas DNA extracted from cells homogenized using the other treatments displayed substantial smearing and lacked a clear HMW DNA band, consistent with high DNA fragmentation (Fig.
Cascade Binding Affinity Assay
Molecular Cloning and DNA Analysis
Optimized DNA Extraction for PCR-free Sequencing
Characterizing QD-Peptide Biosensor Conjugates
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