Washed platelets (200 μl) at a concentration of 7.5×106/ml were allowed to spread on 8-chamber glass tissue-culture slides coated with 3 μg/ml Fg or 1% BSA for 1 hour at 37°C. Supernatants were collected and spun at 800 g for 5 minutes. The concentration of PF4 in each sample as determined using the Mouse CXCL4/PF4 Quantikine ELISA kit (R&D systems).
Mouse cxcl4 pf4 quantikine elisa kit
Manufactured by R&D Systems
The Mouse CXCL4/PF4 Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure mouse CXCL4/PF4 levels in cell culture supernates, serum, and plasma.
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Lab products found in correlation
2 protocols using mouse cxcl4 pf4 quantikine elisa kit
1
Platelet Spreading and PF4 Quantification
2
Quantitative ELISA Assays for Chemokines
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ELISA assays were done according to manufacturer instructions by using the mouse CXCL1/KC quantikine ELISA kit (R&D, cat. no. MKC00B), the mouse CXCL4/PF4 quantikine ELISA kit (R&D, cat. no. MCX400), the mouse CXCL5/LIX quantikine ELISA kit (R&D, cat. no. MX000), and the RayBio mouse CXCL7/TCK-1 ELISA Kit (RayBiotech, cat. no. ELM-TCK1-1).
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