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4 protocols using donkey anti rabbit na934

1

Endothelial Barrier Regulation: Molecular Insights

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Reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad Laboratories (Hercules, CA, USA). All Quantikine ELISA kits were purchased from R & D systems. The antibodies used in this study were obtained from various sources: rabbit anti-ZO-1 (#402200), mouse anti-Occludin (#331500), and anti-Claudin-5 (#4C3C2) from Life Technologies; mouse anti-PECAM-1 (#sc-376764), mouse anti-VCAM-1 (#sc-13160), rabbit anti-Nrf2 (#sc-722), mouse anti-NQO-1 (#sc-376023), mouse anti-HO1 (#sc-390991), and mouse anti-NFκB-p65 (#sc-(F-6)-8008) from Santa Cruz Biotechnology. Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) HRP-linked secondary antibodies were obtained from GE Healthcare (Piscataway, NJ, USA).
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2

Rosiglitazone Modulates Endothelial Barrier

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Rosiglitazone (RSG # A00183, MW: 357.4) was obtained from the Adipogen. Reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad Laboratories (Hercules, CA, USA). The antibodies used in this study were purchased from the various sources: Rabbit anti-ZO-1 (#402200), mouse anti-occludin (#331500) and anti-claudin-5 (#4C3C2) from Life Technologies; rabbit anti-PPARγ (#331500) from Invitrogen; rabbit anti-Nrf2 (#NBP-1-32822) from Novus Biologicals. Mouse anti-PECAM-1 (#sc-376764), mouse anti-NQO-1 (#sc-376023), mouse anti-HO1 (#sc-390991) and mouse anti-NFκB-p65 (#sc-(F-6)-8008) from Santa Cruz Biotechnology. Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) HRP-linked secondary antibodies were obtained from GE Healthcare (Piscataway, NJ, USA).
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3

Mitoprobe JC-1 Assay Protocol

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Mitoprobe JC-1 kit was purchased from Life Technologies Corporation (Carlsbad, CA); rabbit anti-Slc40al (#NBP1–21502), rabbit anti-Abcb6 (#NBP2–58327), mouse anti-Gstpl (#NB110–60512) and rabbit anti Gsta4 (#H00002941-D01P) were purchased from Novus Biologicals (Littleton, CO). Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor” 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). RNeasy Plus Universal Mini Kit (#73404) were purchased from RNAeasy plus mini kit (Qiagen Inc, Germantown, MD). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA). RNA Stabilization Reagent (RNA later, cat #76104).
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4

Sulforaphane Regulation of Nrf2 and NQO1

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L-Sulforaphane (SFN) was purchased from Sigma-Aldrich (St. Louis, MO). Rabbit anti-Nrf2 (#sc-722) and mouse anti-Nqo1 (#sc-376023) antibodies were from Santa Cruz Biotechnology (Dallas, TX); rabbit anti-Slc40a1 was from Novus Biologicals (Littleton, CO). Mouse anti-Glut1 (clone 5B12.3) from EMD Millipore (Billerica, MA); Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor® 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA).
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