Au5812

Manufactured by Beckman Coulter

Sourced in United States

The AU5812 is a fully automated chemistry analyzer system designed for high-volume clinical laboratories. It features a compact, modular design and offers a range of analytical capabilities, including photometric and ion-selective electrochemical detection methods. The AU5812 is capable of performing a wide variety of routine and specialized clinical chemistry tests with high throughput and reliability.

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Lab products found in correlation

Vacutainer, by BD (2 mentions) Quantikine elisa kit, by R&D Systems (2 mentions) Multiplex elisa, by Mesoscale (1 mentions)

Spelling variants of this product

AU5812 Clinical Chemistry Analyzer (3 citations)

5 protocols using au5812

Cardiovascular Risk Biomarkers Measurement

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During the 6-month visit, participants were interviewed, and measurements of BP, weight, height, and hip and waist circumferences obtained. Fasting blood samples for lipids, glucose, and hsCRP were collected into evacuated blood collection tubes with ethylenediaminetetraacetic acid anticoagulant (BD Vacutainer), centrifuged while refrigerated, placed into an ice bath at 2–4 °C, and then kept frozen at –80 °C at the University of Nairobi Laboratory in Kenya. Samples were later batched and shipped for testing by the Department of Laboratory Medicine Research Testing Service at the University of Washington in Seattle, USA. The specimens were thawed, mixed thoroughly, and then analyzed using the Beckman Coulter AU5812 automated chemistry and immunochemistry analyzer for lipids, glucose, and hsCRP. For hsCRP, the analyzer measured the rate of decrease in light intensity transmitted (increase in absorbance) through particles suspended in solution as a result of complexes formed during the antigen-antibody reaction between the CRP of the patient serum and the rabbit anti-CRP antibodies coated on latex particles.

Osoti A.O., Page S.T., Richardson B.A., Guthrie B.L., Kinuthia J., Polyak S.J, & Farquhar C. (2020). Postpartum metabolic syndrome and high-sensitivity C-reactive protein after gestational hypertension and pre-eclampsia. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 151(3), 443-449.

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Cardiometabolic Biomarkers in Adipose Tissue

Cited 1 time

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Serum levels of IL-1β, IL-6 and TNF-α were measured using multiplex ELISA (Meso-Scale Discovery, Rockville, Maryland, USA). Samples with a coefficient of variation greater than 30% were rerun and the duplicate and the lower coefficient of variation was averaged for the analyses. hsCRP testing was performed using an automated Beckman Coulter AU5812. Serum levels of intestinal fatty acid binding protein (I-FABP), sCD163 and sCD14 were measured using commercially available ELISA assays (Quantikine ELISA kit; R&D Systems, Minneapolis, Minnesota, USA). The inter-assay coefficients of variation were less than 11%. All samples were tested centrally at University of Washington (Seattle, Washington, USA). Assays were performed in duplicate and in accordance with manufacturers’ protocols. These markers were selected because they have been shown to be associated with cardiometabolic diseases or death and were likely to be produced by adipose tissue-resident immune cells [34 (link)–37 (link)].

Temu T.M., Wagoner J., Masyuko S., O’Connor A., Zifodya J.S., Macharia P., Wanjalla C.N., Mogaka J.N., Chohan B., Omodi V.M., Gervassi A.L., Oyugi J., Page S.T., Farquhar C, & Polyak S.J. (2021). Central obesity is a contributor to systemic inflammation and monocyte activation in virally suppressed adults with chronic HIV in Kenya. AIDS (London, England), 35(11), 1723-1731.

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Serum CK Activity Measurement

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Blood was drawn by a cardiac puncture. Serum was separated by centrifugation (1,500 rpm, 5 min) and stored at −80°C. The serum CK activity was measured with CK-Nac reagent (Beckman Coulter, Brea, CA, USA) using automated chemistry analyzer (AU5812; Beckman Coulter).

Seo K., Kim E.K., Choi J., Kim D.S, & Shin J.H. (2021). Functional recovery of a novel knockin mouse model of dysferlinopathy by readthrough of nonsense mutation. Molecular Therapy. Methods & Clinical Development, 21, 702-709.

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Fasting Lipid and Glucose Measurements

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Study subjects underwent structured interviews, measurements of BP, weight, height and WC and blood draws. Blood samples were collected from the antecubital vein 9–12 hours after fasting into evacuated blood collection tubes with ethylenediaminetetraacetic acid anticoagulant (BD Vacutainer®) for lipids or a serum separator gel for glucose. Samples were inverted gently 5–10 times, stored on ice, centrifuged in a refrigerated centrifuge at 1,500 rpm for 30 minutes to isolate the plasma fraction at 4°C and then placed into an ice bath at 2–4°C within 2 hours of collection. Plasma and serum aliquots were kept frozen at −80°C at the University of Nairobi laboratory, then batched and shipped to Seattle, USA for testing by the University of Washington Department of Laboratory Medicine’s Research Testing Service. On the day of analysis, the specimens were thawed and mixed thoroughly. Serum lipids and glucose were quantified using the Beckman Coulter AU5812 automated chemistry and immunochemistry analyzer using enzymatic assays.

OSOTI A.O., PAGE S.T., RICHARDSON B.A., GUTHRIE B.L., KINUTHIA J., POLYAK S.J, & FARQUHAR C. (2019). Postpartum metabolic syndrome after gestational hypertension and preeclampsia, a prospective cohort study. Pregnancy hypertension, 18, 35-41.

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Profiling Cardiometabolic Biomarkers

Cited 1 time

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Serum levels of IL-1β, IL-6, and TNF-α were measured using multiplex enzyme-linked immunosorbent assay (Meso-Scale Discovery, Rockville, MD). Samples with a coefficient of variation greater than 30% were rerun and the duplicate and the lower coefficient of variation was averaged for the analyses. hsCRP testing was performed using an automated Beckman Coulter AU5812. Serum levels of intestinal fatty acid binding protein (I-FABP), sCD163 and sCD14 were measured using commercially available ELISA assays (Quantikine ELISA kit, R&D systems). The inter-assay coefficients of variation were <11%. All samples were tested centrally at University of Washington (Seattle, WA). Assays were performed in duplicate and in accordance with manufacturers’ protocols. These markers were selected because they have been shown to be associated with cardiometabolic diseases or death and were likely to be produced by adipose tissue-resident immune cells^{[34 (link)–37 (link)]}.

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