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2 protocols using para fluorofentanyl

1

Fluorofentanyl Metabolism in Human Hepatocytes

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Ortho-, meta- and para-fluorofentanyl were purchased from Cayman Chemicals (Ann Arbor, MI, USA). LC-MS grade acetonitrile, formic acid and methanol were purchased from Fisher Scientific (Gothenburg, Sweden). Ammonium formate was obtained from Sigma–Aldrich (Stockholm, Sweden) and 99.5% ethanol from Kemetyl (Haninge, Sweden). Divide; Cryo-preserved human hepatocytes (LiverPool™, 10-donor-pool, Lot nr. RBR) and InVitro Gro HT medium were from BioreclamationIVT (Baltimore, MD, USA). Williams medium E (without L-glutamine and phenol red), L-glutamine 200 mM and Hepes 1 M buffer solution from Gibco® by life technologies™ were purchased from Thermo (Stockholm, Sweden). MilliQ Gradient 10 production unit from Millipore (Billerica, MA, USA) was used to produce high-purity water. β-Glucuronidase/arylsulfatase stock solution (Helix promatia), with activities of 4.5 and 14 U/ml respectively, was purchased from Roche Diagnostics (Mannheim, Germany).
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2

Quantitative Analysis of Synthetic Opioids

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Standard of acetylfentanyl (hydrochloride), acrylfentanyl (hydrochloride), alfentanil (hydrochloride), butyrylfentanyl, carfentanyl, (±)-cis-3-methylfentanyl (hydrochloride), (±)-cis-3-methylthiofentanyl (hydrochloride), fentanyl (hydrochloride), furanylfentanyl (hydrochloride), α-methylfentanyl (hydrochloride), α-methylthiofentanyl (hydrochloride), β-hydroxyfentanyl (hydrochloride), ortho-fluorofentanyl (hydrochloride), ocfentanyl, para-fluorofentanyl (hydrochloride), remifentanyl (hydrochloride), sufentanyl, 4-ANPP, norfentanyl, butyrylfentanyl carboxy metabolite, despropionylpara-fluorofentanyl, (±)-trans-3-methyl norfentanyl, methoxyacetyl norfentanyl (hydrochloride), valerylfentanyl carboxy metabolite, morphine, methadone, EDDP, 6-MAM, codeine, MT-45, fentanyl-d5 (hydrochloride) and nordentanyl-d5 were purchased from Cayman Chemical (Ann Arbor, MI, USA) in form of methanolic solution at the concentration of 1 mg mL−1. Working solutions were prepared at the concentration of 1 μg mL−1 and stored at −20 °C. Water, methanol, acetonitrile and 2-propanol were obtained from Carlo Erba (Milano, Italia) while sodium carbonate, sodium bicarbonate and ammonium formate were purchased from Sigma-Aldrich (Milwaukee, WI, USA).
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