The RAW264.7 cells were stimulated with LPS (1 µg/mL) for 18 h and then treated with clusterin or TFF3 (5 µg/mL) for 18 h. We measured the TNF-α levels in supernatants using ELISA kits (MEIMIAN, MM-0180R1, Jiangsu, China) as per the manufacturer’s instructions.
Mm 0180r1
Manufactured by Jiangsu Meimian
Sourced in China
The MM-0180R1 is a laboratory equipment product. It serves as a core component for conducting various scientific experiments and analyses. The detailed specifications and intended use of this product are not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Mm 0190r1, by Jiangsu Meimian (2 mentions)
Lipopolysaccharides (lps), by MedChemExpress (1 mentions)
A003 1 2, by Nanjing Jiancheng (1 mentions)
A044 1 1, by Nanjing Jiancheng (1 mentions)
A001 3 2, by Nanjing Jiancheng (1 mentions)
A007 1 1, by Nanjing Jiancheng (1 mentions)
Mm 0047r1, by Jiangsu Meimian (1 mentions)
3 protocols using mm 0180r1
1
Clusterin and TFF3 Modulate LPS-Induced TNF-α
2
Anti-oxidative and Anti-inflammatory Assays
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Assay kits for SOD, MDA, CAT, GPx, and MPO were obtained from Jiancheng Bioengineering Institute (A001-3-2, A003-1-2, A007-1-1, H545-1-1, and A044-1-1) (Nanjing, China). ELISA assays for PGI, PGII, iNOS, NO, PGE2, TNF-α, IL-1β, and IL-6 were obtained from MEIMIAN (MM-70280R1, MM-70274R1, MM-0889R1, MM-70810R2, MM-0068R1, MM-0180R1, MM-0047R2, and MM-0190R1). Rabbit anti-NF-κBp65 conjugated antibody (AF5006), rabbit anti-p-NF-κBp65 conjugated antibody (AF2006), and mouse anti-β-actin antibody (T0022) were obtained from Affinity. Lipopolysaccharides (LPS) (batch number HY-D1056) were obtained from MedChemExpress.
3
Serum Biomarkers of Ischemic Stroke in MCAO Rats
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The serum levels of endothelin-1 (ET-1) (MM-0560R1; MEIMIAN, Jiangsu, China), nitrous oxide (NO) (MM-20607R1; MEIMIAN, Jiangsu, China), interleukin-1β (IL-1β) (MM-0047R1; MEIMIAN, Jiangsu, China), IL-6 (MM-0190R1; MEIMIAN, Jiangsu, China), tumor necrosis factor-α (TNF-α) (MM-0180R1; MEIMIAN, Jiangsu, China), endothelial nitric oxide synthase (eNOS) (RX300651R; RUIXING, Fujian, China), malonaldehyde (MDA) (S0131S; Biyuntian Biotechnology Co., LTD. Jiangsu, China), glutathione (GSH) (S0053; Biyuntian Biotechnology Co., LTD. Jiangsu, China), and superoxide dismutase (SOD) (S0101S; Shanghai Biyuntian Biotechnology Co., LTD. China) were tested using the ELISA kits following the manufacturer’s instructions. The serum creatinine (Scr) and blood urea nitrogen (BUN) levels of cilostazol in MCAO rats were determined using an automatic biochemical analyzer.
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