Colloidal gold particles

Manufactured by Merck Group

Colloidal gold particles are a suspension of sub-microscopic gold particles in a liquid medium. They are characterized by their small size, typically ranging from 2 to 100 nanometers in diameter. Colloidal gold particles exhibit unique optical properties due to their size and surface plasmon resonance, which can be utilized in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai f30 transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Us1000 ccd camera, by Thermo Fisher Scientific (1 mentions) Tecnai f20, by Thermo Fisher Scientific (1 mentions)

4 protocols using colloidal gold particles

Dual-axis Electron Tomography of Mitotic Spindles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Colloidal gold particles (15 nm; Sigma-Aldrich) were attached to both sides of semi-thick sections collected on copper slot grids to serve as fiducial markers for subsequent image alignment. For dual-axis electron tomography58 (link), series of tilted views were recorded using a TECNAI F30 transmission electron microscope (FEI Company, Eindhoven, The Netherlands) operated at 300 kV. Images were captured every 1° over a ±60° range and a pixel size of 2.3 nm using a Gatan US1000 CCD camera (2k × 2k). For each serial section two montages of 2 × 3 frames were collected and combined to a supermontage using the IMOD software package to cover the pole-to-pole distance of the spindles59 (link). For image processing, the tilted views were aligned using the positions of the Colloidal gold particles as fiducial markers. Tomograms were computed for each tilt axis using the R-weighted back-projection algorithm60 (link). For double-tilt data sets two montages, each consisting of six tomograms, were aligned to each other and combined to a supermontage58 (link). To cover a large volume of the pole-to-pole region of each mitotic spindle, we recorded on average 24 consecutive serial sections per spindle.

Redemann S., Baumgart J., Lindow N., Shelley M., Nazockdast E., Kratz A., Prohaska S., Brugués J., Fürthauer S, & Müller-Reichert T. (2017). C. elegans chromosomes connect to centrosomes by anchoring into the spindle network. Nature Communications, 8, 15288.

+ Open protocol

+ Expand

Electron Tomography of Mitotic Spindles

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prior to imaging, 15 nm colloidal gold particles (Cat# 777137, Sigma-Aldrich) were added to both sides of the 300 nm semi-thick sections to serve as fiducial markers for the calculation of electron tomograms. Next, a series of tilted images was taken by using a TECNAI F30 transmission electron microscope (Thermo Fisher Scientific) operated at 300 kV. TEM images were taken every 1° over a ±60° range using a Gatan US1000 CCD camera (2k × 2k). The pixel size of the images was 2.3 nm. Tilted views/images were aligned using the fiducial gold markers and later back-projected and combined to a super montage using IMOD software package or 3dmod software package ^{80 (link)}. To cover the pole-to-pole region of each mitotic spindle, on average, 24 consecutive serial sections per spindle were imaged and processed accordingly ^{46 (link)}.

Haddad A, & Turkewitz A.P. (1997). Analysis of exocytosis mutants indicates close coupling between regulated secretion and transcription activation in Tetrahymena. Proceedings of the National Academy of Sciences of the United States of America, 94(20), 10675-10680.

+ Open protocol

+ Expand

Dual-Axis Electron Tomography Reconstruction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Colloidal gold particles (15 nm; Sigma-Aldrich) were attached to both sides of semi-thick sections collected on copper slot grids to serve as fiducial markers for subsequent image alignment. For dual-axis electron tomography, a series of tilted views were recorded using a TECNAI F20 transmission electron microscope (FEI Company, Eindhoven, The Netherlands) operated at 200 kV. Images (4K x 4K) were captured every 1h over a ±60 range and a pixel size of 2.3nm using a TVIPS CCD camera. We used the IMOD software package (http://bio3d.colourado.edu/imod) for image processing, which contains all the programs needed for calculating electron tomograms. The tilted views were aligned using the positions of the Colloidal gold particles as fiducial markers. Tomograms were computed for each tilt axis using the R-weighted back-projection algorithm. Reconstructed tomograms were flattened.

Rao V.G., Subramanianbalachandar V., Magaj M.M., Redemann S, & Kulkarni S.S. (2023). Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivo. bioRxiv.

+ Open protocol

+ Expand

Colloidal Gold Fiducial Markers for Electron Tomography

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mauro M.S., Celma G., Zimyanin V., Magaj M.M., Gibson K.H., Redemann S, & Bahmanyar S. (2022). Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth. eLife, 11, e75513.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!