Rosa tdtomato egfp rosamtmg

C57BL6/J, Prx1^Cre,⁽²⁶⁾Prx1^CreERT (Stock number #029211),⁽²⁷⁾Pdgfra^CreERT (Stock number # 018280),⁽²⁸⁾ Rosa‐tdTomato‐EGFP (Rosa^mTmG) and Rosa^LacZ were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). Bmpr1a^fl/fl mice were provided by Dr. Yuji Mishina (University of Michigan, Ann Arbor, MI, USA).⁽²⁹, ³⁰⁾ All strains were maintained on a C57BL6/J background. Mice were bred and kept under controlled pathogen conditions in separated ventilated cages with controlled humidity and ambient temperature, with 12:12‐hour light:dark cycles and free access to food and water in the animal facilities of IMRB, Creteil, and Imagine Institute, Paris. All experiments were performed in compliance with procedures approved by the Paris Est Creteil and Paris University Ethical Committees. Both males and females were used in all experiments. For in vitro experiments, 4‐week‐old to 8‐week‐old mice were used, and for in vivo experiments 12‐week‐old to 14‐week‐old mice were used. No specific randomization methods were used. Sample labeling allowed blind analyses.

C57BL/6ScNj, Prx1^Cre, and Rosa-tdTomato-EGFP (Rosa^mTmG) transgenic mice were obtained from Jackson Laboratory (Bar Harbor, ME). Fgfr3^Y367C mice were generated in Dr. Legeai-Mallet’s laboratory (Pannier et al., 2009 (link)). In brief, the point mutation was inserted within exon 9 at position 367 of the Fgfr3 gene, directly followed by a floxed neo cassette. Under Cre recombination, the neo cassette is removed, allowing expression of the mutated allele of the Fgfr3 gene. Mice were bred and genotyped in our laboratory using primers purchased from Eurofins (Eurofins Scientific, Lux). Five- to 8-week-old mice were used for in vitro experiments and 3 month-old mice for in vivo experiments. Male and female mice were used and distributed homogenously within experimental groups. All procedures were approved by the Paris University and Creteil University Ethical Committees.