Following overnight fasting, a 12 ml blood sample was collected by venipuncture. Whole blood was immediately centrifuged and serum was tested for glucose using a glucose oxidase phenol 4-aminoantipyrine peroxidase kit (Randox, Crumlin, UK) and a Hitachi 7600 analyzer (Hitachi; Tokyo, Japan). Serum insulin was tested using radioimmunology assay kit (North Institute of Biological Technology; Beijing, China) using a XH-6020 gamma counter (North Institute of Biological Technology). Whole blood HbA1c high performance liquid chromatography analysis (model HLC-723 G7; Tosoh Corporation, Tokyo, Japan) generated continuous outcomes for fasting glucose, HbA1c, insulin and natural log transformed HOMA-IR, calculated as (insulin × glucose/6.945 pmol/l) [26 (link), 27 (link)].
Glucose oxidase phenol 4 aminoantipyrine peroxidase kit
Manufactured by Randox
Sourced in United Kingdom
The Glucose oxidase phenol 4-aminoantipyrine peroxidase kit is a reagent used for the quantitative determination of glucose in biological samples. It utilizes the enzymatic reaction between glucose, glucose oxidase, and peroxidase to produce a colored complex that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using glucose oxidase phenol 4 aminoantipyrine peroxidase kit
1
Fasting Glucose, Insulin, and HbA1c Assay
2
Comprehensive Metabolic Panel in Fasting Individuals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fasting blood samples after an overnight fast of at least 8 h were collected in both study visits of 2015 and 2018. Serum glucose was measured using the glucose oxidase phenol 4-aminoantipyrine peroxidase kit (Randox Laboratories Ltd, UK). Hemoglobin A1c (HbA1c) was measured by high-performance liquid chromatography system (HLC-723 G7, Tosoh Co., Japan). Fasting insulin was determined by radioimmunology in a gamma counter using an XH-6020 analyzer (North Institute of Bio-Tech, China). Serum high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), and triglycerides (TG) were measured using the glycerol-phosphate oxidase method and polyethylene glycol (PEG)- modified enzyme assay (Kyowa Medex Co., Ltd, Japan) on the automatic analyzer (Hitachi 7600, Hitachi Inc., Japan). High-sensitivity C-reactive protein (CRP) was measured by the immunoturbidimetric method with commercial reagents (Denka Seiken, Japan) on an automatic analyzer (Hitachi 7600, Hitachi Inc., Japan).
3
CHNS Fasting Blood Metabolic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the 2009 survey of CHNS, overnight fasting blood were collected from the participants.
The whole blood was centrifuged immediately after collection, and stored at -86 °C for future measurement. Serum glucose was measured using a glucose oxidase phenol 4aminoantipyrine peroxidase kit (Randox) and a Hitachi 7600 analyzer (Hitachi, Tokyo, Japan). Serum insulin was tested using a XH-6020 gamma counter (North Institute of Biological Technology, Beijing, China) with radioimmunology assay kit (North Institute of Biological Technology, Beijing, China). Whole blood HbA1c was detected with high performance liquid chromatography (model HLC-723 G7; Tosoh Corporation, Tokyo, Japan).
The homeostasis model assessment of insulin resistance (HOMA-IR) was estimated as: HOMA-IR =glucose (mmol/L) × insulin (µU/mL) /22.5.
The whole blood was centrifuged immediately after collection, and stored at -86 °C for future measurement. Serum glucose was measured using a glucose oxidase phenol 4aminoantipyrine peroxidase kit (Randox) and a Hitachi 7600 analyzer (Hitachi, Tokyo, Japan). Serum insulin was tested using a XH-6020 gamma counter (North Institute of Biological Technology, Beijing, China) with radioimmunology assay kit (North Institute of Biological Technology, Beijing, China). Whole blood HbA1c was detected with high performance liquid chromatography (model HLC-723 G7; Tosoh Corporation, Tokyo, Japan).
The homeostasis model assessment of insulin resistance (HOMA-IR) was estimated as: HOMA-IR =glucose (mmol/L) × insulin (µU/mL) /22.5.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!