Insulin glargine

Example 1

5 g of insulin glargine (Gan & Lee Pharmaceuticals) are mixed with the enzyme carboxypeptidase B (Reference 08039852001; Sigma-Aldrich) at pH 8.0 (pH adjusted by addition of Tris buffer), and the mixture is allowed to stand for 17 hours at 25° C., the insulin glargine concentration being approximately 4 mg/mL. The enzyme/glargine ratio is 1/500. The mixture is then purified by liquid chromatography, dialyzed against hydrochloric acid 0.01N and then lyophilized. The result is human insulin A21G with a purity of 98% and a yield of approximately 90%. The molecular weight of the insulin measured by mass spectrometry (Maldi-Tof) is 5752 Da. The human insulin A21G can also be obtained using the recombinant technology as described by Kohn et al. (Peptides 2007, 28, 935-948).

Example 1

5 g of insulin glargine (Gan & Lee Pharmaceuticals) are mixed with the enzyme carboxypeptidase B (Reference 08039852001; Sigma-Aldrich) at pH 8.0 (pH adjusted by addition of Tris buffer), and the mixture is allowed to stand for 17 hours at 25° C., the insulin glargine concentration being approximately 4 mg/mL. The enzyme/glargine ratio is 1/500. The mixture is then purified by liquid chromatography, dialyzed against hydrochloric acid 0.01N and then lyophilized. The result is human insulin A21G with a purity of 98% and a yield of approximately 90%. The molecular weight of the insulin measured by mass spectrometry (Maldi-Tof) is 5752 Da. The human insulin A21G can also be obtained using the recombinant technology as described by Kohn et al. (Peptides 2007, 28, 935-948).