The QDs were prepared for transmission electron microscopy (TEM) by placing a drop of suspended QDs onto a copper grid coated with a holey carbon support film (Agar Scientific Ltd) and plunge frozen in liquid ethane followed by freeze drying preserving the original features of the QDs (Hondow et al., 2012 ). Images were subsequently captured. Images were collected by an FEI Tecnai TF20 FEG-TEM operating at 200 kV fitted with a Gatan Orius SC600A camera and an Oxford Instruments INCA 350 energy dispersive x-ray (EDX) system with an 80 mm2 X-Max SDD detector.
Tecnai tf20 feg tem
The Tecnai TF20 FEG-TEM is a field emission gun transmission electron microscope (FEG-TEM) designed for high-resolution imaging and analysis of materials at the nanometer scale. It features a high-brightness electron source, advanced optics, and a sophisticated control system to enable precise and stable operation.
2 protocols using tecnai tf20 feg tem
Characterization of Quantum Dots
The QDs were prepared for transmission electron microscopy (TEM) by placing a drop of suspended QDs onto a copper grid coated with a holey carbon support film (Agar Scientific Ltd) and plunge frozen in liquid ethane followed by freeze drying preserving the original features of the QDs (Hondow et al., 2012 ). Images were subsequently captured. Images were collected by an FEI Tecnai TF20 FEG-TEM operating at 200 kV fitted with a Gatan Orius SC600A camera and an Oxford Instruments INCA 350 energy dispersive x-ray (EDX) system with an 80 mm2 X-Max SDD detector.
Transmission Electron Microscopy of Cell Samples
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