In vitro maturation was performed under standard conditions for 24 h in TCM 199 Earl’s salt medium supplemented with 10% FCS, 5 μg/mL LH (Lutropin, Vetoquinol, France), 0.5 μg/mL FSH (Folltropin, Vetoquinol), 0.2 mM sodium pyruvate, 5 μg/mL gentamycin, and 1 mg/mL estradiol 17β. Cultures were performed in 70 μL droplets (up to 20 oocytes/droplet) of the medium under mineral oil, at 38.5°C in 5% CO2.
Lutropin
Manufactured by Vetoquinol
Sourced in France
Lutropin is a laboratory equipment product manufactured by Vetoquinol. It is designed to measure the levels of luteinizing hormone (LH) in biological samples. LH is a hormone that plays a crucial role in the regulation of reproductive processes in various species.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lutropin
1
In vitro Oocyte Maturation Protocol
2
Bovine Oocyte Maturation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ovaries were collected from slaughtered Holstein-Friesian cows (Bos taurus) whose age, genealogy, and physiological status were unknown. Ovaries were transported in sterile saline solution (0.9% NaCl) supplemented with 150 mg/L kanamycin and maintained at 30°C. About 5940 oocytes, in twenty replications, were used to produce embryos to submit to IVC with different culture systems. Oocytes were retrieved by aspiration of 3-5 mm diameter follicles with 18 G needles. Cumulus-oocyte complexes (COCs) were selected and washed three times in preincubated TCM 199-Hepes buffered supplemented with 10% FCS.
In vitro maturation was performed for 24 h in TCM 199 Earl's Salt medium supplemented with 10% FCS, 5 μg/mL LH (Lutropin, Vetoquinol, France), 0.5 μg/mL FSH (Folltropin, Vetoquinol), 0.2 mM sodium pyruvate, 5 μg/mL gentamycin and 1 mg/mL estradiol 17β. Cultures were performed in 70 μL droplets (up to 20 oocytes/droplet) of the medium under mineral oil, at 38.5°C in 5% CO2.
In vitro maturation was performed for 24 h in TCM 199 Earl's Salt medium supplemented with 10% FCS, 5 μg/mL LH (Lutropin, Vetoquinol, France), 0.5 μg/mL FSH (Folltropin, Vetoquinol), 0.2 mM sodium pyruvate, 5 μg/mL gentamycin and 1 mg/mL estradiol 17β. Cultures were performed in 70 μL droplets (up to 20 oocytes/droplet) of the medium under mineral oil, at 38.5°C in 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!