The 27HC (purity ≥ 99.9%) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and was dissolved in absolute ethanol at a stock concentration of 20 mM and then stored at -80°C. C646 was purchased from MedChem Express (MCE, USA) and was dissolved in dimethyl sulfoxide (DMSO) at a stock concentration of 10 mM and then stored at -80°C. N-Acetylcysteine (NAC) (an antioxidant) was purchased from Beyotime Co., Ltd. (China). REV was purchased from Xuhuang Biotechnology Co., Ltd. (Xi'an, China). Azacitidine (AZA) and S-adenosyl methionine (SAM) were purchased from Sigma-Aldrich. All other reagents were of the highest analytical grade available. Anti-Ac-STAT3Lys685 (2523), anti-SIRT1 (8469), anti-DNMT1 (5032), and anti-p-STAT3Tyr705 (9145) were purchased from Cell Signaling Technology (CST, USA).
BV2 microglial cells and PC12 rat pheochromocytoma cells were purchased from the Institute of Basic Medical Sciences of the China Science Academy. The cells were maintained in high glucose Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 mg/ml streptomycin. They were maintained in a humidified 5% CO2/95% air environment at 37°C.
BV2 microglial cells and PC12 rat pheochromocytoma cells were purchased from the Institute of Basic Medical Sciences of the China Science Academy. The cells were maintained in high glucose Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 mg/ml streptomycin. They were maintained in a humidified 5% CO2/95% air environment at 37°C.