The levels of NADPH and glutathione (GSH) in cultured HAPI microglial cells were measured with an NADPH/NADP+ kit (Beyotime) and a GSH/oxidized GSH (GSSG) kit (BioAssay Systems, San Francisco, CA, USA) following the manufacturer’s instructions. The ROS levels in HAPI microglial cells were detected using dihydroethidium staining (1 μM; Sigma, St. Louis, MO, USA) as previously described (Cao et al., 2017).
Nadph nadp kit
Manufactured by Beyotime
Sourced in United States, China
The NADPH/NADP+ kit is a tool for the quantitative determination of the reduced and oxidized forms of nicotinamide adenine dinucleotide phosphate (NADPH and NADP+) in samples. It is a colorimetric assay that allows for the rapid and accurate measurement of these important cellular metabolites.
Automatically generated - may contain errors
Lab products found in correlation
Dihydroethidium, by Merck Group (1 mentions)
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Primerstar hs dna polymerase, by Takara Bio (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Restriction endonuclease, by Thermo Fisher Scientific (1 mentions)
Dna purification kit, by Tiangen Biotech (1 mentions)
Realmastermix kit, by Tiangen Biotech (1 mentions)
Rna extraction kit, by BioFlux (1 mentions)
2 protocols using nadph nadp kit
1
Quantifying Redox Homeostasis in Microglia
2
Molecular Biology Techniques for Cellular Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Plasmids and genomic DNA were extracted using the Plasmids mini kit and DNA purification kit (Tiangen, Beijing, China), respectively. PrimerSTAR HS DNA polymerase (Takara, Dalian, China) was used for PCR. Restriction endonucleases and T4 DNA ligase were purchased from Thermo Scientific (Waltham, USA). Total RNA was extracted using RNA extraction kit (Bio Flux, Beijing, China). The RNA was reversely transcribed into cDNA using the Revert Aid™ First Strand cDNA synthesis kit (Fermentas, Shanghai, China). RT-PCR was performed using the Real Master Mix kit (Tiangen, Beijing, China). The intracellular concentration of NADPH/NADP+ was determined by using NADPH/NADP+ Kit (Beyotime Biotechnology, Shanghai, China).
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