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Transwell chambers with 8.0 m pore polycarbonate membrane inserts

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Transwell chambers with 8.0 μm pore polycarbonate membrane inserts are a type of cell culture insert. The insert features a polycarbonate membrane with 8.0 μm pores that allows the passage of cells and molecules between the upper and lower chambers.

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Lab products found in correlation

Matrigel, by Corning (1 mentions) Crystal violet, by Avantor (1 mentions) Inverted phase contrast microscope, by Olympus (1 mentions) Paraformaldehyde (pfa), by Sinopharm (1 mentions) Giemsa solution, by Merck Group (1 mentions)

Close spelling variants of this product

Transwell chambers (4249 citations) Transwell inserts (2103 citations) Transwell membranes (159 citations) Polycarbonate membrane (137 citations) Transwell insert chambers (136 citations) Transwell polycarbonate membranes (31 citations) Polycarbonate transwell inserts (21 citations) 8 µm transwell chamber (18 citations) Chamber inserts (18 citations) Pore transwells (4 citations)

3 protocols using transwell chambers with 8.0 m pore polycarbonate membrane inserts

1

Proliferation, Migration, and Invasion Assays

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Plate colony formation assay was used to assess ability of cell proliferation. BCAT2‐OE, BCAT2‐KD, and negative control human bladder cancer cells were cultured into 6‐well plates per well. After incubating in 37 °C humidified atmosphere for 2 weeks, colonies were fixed and dyed by paraformaldehyde and crystal violet, respectively.
Transwell chambers with 8.0 µm pore polycarbonate membrane inserts (Corning, USA) were employed to evaluate capacity of cell migration and invasion in vitro. 1 × 105 stable transfected cells were suspended in serum‐free medium and seeded into top chamber with or without Matrigel (Corning, USA), for invasion and migration assay separately. After incubating for 24 h (migration assay) and 48 h (invasion assay), cells adhering to lower surface of membrane were fixed and stained. Cells remained in top chamber were removed by swabs. Five random fields were selected under microscope to calculate cell numbers.
Cai Z., Chen J., Yu Z., Li H., Liu Z., Deng D., Liu J., Chen C., Zhang C., Ou Z., Chen M., Hu J, & Zu X. (2023). BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti‐PD‐1/PD‐L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity. Advanced Science, 10(8), 2207155.
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2

Transwell Assay for Cell Migration

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Transwell chambers with 8.0 µm pore polycarbonate membrane inserts (Corning, Inc.) were placed in 24-well-plates. A total of 4×103 normal or transfected HUVECs were suspended in serum-free M199 medium and seeded into the upper chamber, and 800 µl medium supplemented with 20% FBS was added to the lower chamber. Cells were cultured under normoxic or hypoxic conditions at 37°C for 24 h and washed twice with PBS. Cells that had migrated through the filter were fixed with 4% paraformaldehyde (Sinopharm Chemical Reagent Co., Ltd.) for 20 min and stained using 0.1% crystal violet (Amresco, LLC) for 5 min at room temperature. The stained cells were visualized using an inverted phase contrast microscope (magnification, ×200) (Olympus Corporation), and the number of cells in 5 randomly chosen fields was counted.
Zhao X., Wei X., Wang X, & Qi G. (2020). Long non-coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR-590-3p under hypoxic conditions. Molecular Medicine Reports, 21(6), 2560-2570.
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3

Evaluating HT-29 Cell Migration in 3D Spheroids

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The migration of HT-29 cells in a 3D condition (spheroids) was evaluated using a Transwell migration assay. To this end, Transwell chambers with 8.0 µm pore polycarbonate membrane inserts (Corning Inc.; Corning, NY, USA) were used. After the treatment of HT-29 spheroids with Gemini-Cur NPs, a single-cell suspension was made using the Trypsin-EDTA solution. Then, 200 µl of serum-free medium containing 2 × 10 4 cells was transferred into inserted. The basolateral chamber was filled with 700 μl of culture medium enriched with 1-2% FBS. Plates were kept at 37 °C for 24 h. Thereafter, the number of migrated cells at the bottom surface was counted in 5 random fields. To better identify the migrated cells at the bottom of the plates, we fixed the cells with precooled methanol solution for 10 min and stained with Giemsa solution (Sigma-Aldrich; St. Louis, MO).
Zibaei Z., Babaei E., Zamani A., Rahbarghazi‬ R., & Azeez H.J. (2021). Curcumin-enriched Gemini surfactant nanoparticles exhibited tumoricidal effects on human 3D spheroid HT-29 cells in vitro. Cancer Nanotechnology, 12(1).
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