Cd123 fitc

Manufactured by BD

Sourced in United States

The CD123-FITC is a fluorescent-labeled antibody that binds to the CD123 antigen expressed on the surface of certain cell types. It is designed for use in flow cytometry applications to identify and quantify CD123-positive cells within a sample.

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Lab products found in correlation

Cd11c apc, by BD (2 mentions) Cd80 fitc, by BD (1 mentions) Cd86 pe, by BD (1 mentions) Hla dr apc cy7, by BD (1 mentions) Dc sign pe, by R&D Systems (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Facs lyse, by BD (1 mentions) Cd80 pe, by BD (1 mentions) Cd20 apc cy7, by BD (1 mentions) Cd3 a700, by BD (1 mentions)

Spelling variants of this product

CD123-PE/PE-Cy5/FITC (4 citations) CD123-FITC (clone 7G3), (2 citations)

2 protocols using cd123 fitc

Comprehensive Immune Cell Profiling by Flow Cytometry

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The following Abs were used for flow cytometry: CD80-FITC, CD86-PE, CD14-Pacific blue, Linage-Alexa700 (CD3, CD19, CD16), HLA-DR-APC-cy7, CD11c-APC, CD123-FITC, CD81-PE (BD Bioscience, USA), DC-SIGN-PE (R&D Systems, USA). A viability dye (Molecular Probes® LIVE/DEAD® Fixable Dead Cell Stain Kits, Invitrogen) was used to exclude dead cells. Cells were stained and analyzed by FACS using the BD LSRII cytometer and FlowJo software.

Zhang Y., El-Far M., Dupuy F.P., Abdel-Hakeem M.S., He Z., Procopio F.A., Shi Y., Haddad E.K., Ancuta P., Sekaly R.P, & Said E.A. (2016). HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses. Scientific Reports, 6, 29447.

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Multicolor Flow Cytometry Panel

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200µl of whole blood was stained with a cocktail of antibodies consisting of CD3-A700 (Clone SP34-2, BD Pharmingen), CD20-APC-Cy7 (Clone L27), CD14-PE-Texas Red (Clone RMO52), HLA-DR-PerCP (Clone L243), CD123-FITC (Clone 7G3), CD11C-APC (Clone S-HCL-3) and CD80-PE (Clone L307.4) at room temperature for 30min. A separate panel used the same antibodies except CD80 was replaced with anti-α4β7-PE from NHP reagent resource. RBCs were lysed and lymphocytes were fixed using FACSlyse (BD biosciences, CA) according to manufacturers instructions. Cells were then washed twice with FACS wash (PBS with 2% FBS) and acquired on LSRII flowcytometer (BD biosciences, CA). Approximately, 150,000 lymphocytes were acquired and data was analyzed using the FlowJo software. Lymphocytes were identified based on forward and side scatter, HLA-DR+ dendritic cells (DC) were identified by excluding CD3+ CD20+ CD14+ cells, and CD123+ CD11C− cells were defined as plasmacytoid DC (pDC). All the antibodies were purchased from either BD Biosciences, BD Pharmingen or Beckman Coulter.

Kannanganat S., Wyatt L.S., Gangadhara S., Chamcha V., Chea L.S., Kozlowski P.A., LaBranche C.C., Chennareddi L., Lawson B., Reddy P.B., Styles T.M., Vanderford T.H., Montefiori D.C., Moss B., Robinson H.L, & Amara R.R. (2016). High doses of granulocyte-macrophage colony stimulating factor inhibit antibody responses in rectal secretions and diminish MVA/SIV vaccine protection in TRIM5α restrictive macaques. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3586-3596.

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