Anti micu1

Manufactured by Merck Group

Anti-MICU1 is a laboratory product developed by Merck Group. It is designed to detect and quantify the MICU1 protein, which plays a crucial role in the regulation of mitochondrial calcium uptake. The product offers a reliable and efficient tool for researchers studying mitochondrial function and related biological processes.

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Lab products found in correlation

Anti hsp60, by BD (2 mentions) Anti micu2, by Abcam (2 mentions) Igg fc specific peroxidase, by Merck Group (2 mentions) Complete mini, by Merck Group (2 mentions) Anti smad3, by Cell Signaling Technology (2 mentions) Anti myog, by Santa Cruz Biotechnology (2 mentions) Anti β actin, by Merck Group (2 mentions) β glycerophosphate, by Merck Group (2 mentions) Ab52175, by Abcam (1 mentions) Ab101465, by Abcam (1 mentions) Anti mhc, by Santa Cruz Biotechnology (1 mentions) Anti mcu, by Cell Signaling Technology (1 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (1 mentions) Anti nf κb, by Abcam (1 mentions) Anti p38 mapk, by Cell Signaling Technology (1 mentions) Anti phospho smad3, by Cell Signaling Technology (1 mentions) Anti phospho nf κb, by Cell Signaling Technology (1 mentions) Anti opa1, by BD (1 mentions) 17β estradiol e8875, by Merck Group (1 mentions) Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rabbit anti-MICU1 (2 citations)

4 protocols using anti micu1

Comprehensive Protein Profiling of Cell and Tumor Lysates

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Whole-cell and tumour lysates were isolated using RIPA buffer supplemented with protease inhibitors (Complete Mini, Sigma-Aldrich Inc.) and phosphatase inhibitors including sodium pyrophosphate, β-glycerophosphate, sodium fluoride and sodium orthovanadate (Sigma-Aldrich). The following primary antibodies were used: anti-MCU (#D2Z3B 1:1000, Cell Signaling), anti-MICU1 (#HPA037479 1:1000, Sigma-Aldrich), anti-MICU2 (#ab101465 1:1000, Abcam), anti-phospho-SMAD3 (#C25A9, 1:1000, Cell Signaling), anti-SMAD3 (#9513, 1:1000, Cell Signaling), anti-MYOG (#sc-12732, 1:500, Santa-Cruz), anti-MHC (#sc-32732, 1:250, Santa-Cruz) anti-HSP60 (#611563, BD Biosciences), anti-phospho-NF-κB (#3037, 1:1000, Cell Signaling), anti-NF-κB (#ab52175, 1:500, Abcam), anti-phospho-p38 MAPK (#9211, 1:1000, Cell Signaling), anti-p38 MAPK (#9212, 1:1000, Cell Signaling), and anti-β-actin (#A2228, 1:10,000, Sigma-Aldrich). Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.

Chiu H.Y., Loh A.H, & Taneja R. (2022). Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma. Cell Death & Disease, 13(4), 419.

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Mitochondrial Regulation by Steroid Hormones

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The following antibodies and reagents were used in this study: ATP (Research Product International; A300030), mitoTracker Green FM (Life Technology; M7514), and tetramethylrhodamine methyl ester (Invitrogen; I34361), anti‐MCUa (Prestige Antibodies; HPA016480), anti‐EMRE (Sigma; HPA060340), anti‐MICU1 (Sigma; HPA037480), and anti‐MCUb (Abgent; C109B, AP12355b). Anti‐CoxIV (4850S) and anti‐GAPDH (2178), anti‐phosho‐eNOS (p‐eNOS Ser1177 and Thr495; 9571 and 9574), anti‐eNOS antibodies (9586), anti‐phospho pDRP‐1 (Ser⁶¹⁶, D9A1; 4494), anti‐DRP1 (D6C7, 8570), and anti‐MFN2 (D2D10, 9482) were purchased from Cell Signaling and anti‐OPA1 (612806) from BD Biosciences. 17β‐Estradiol (E8875) and testosterone (T1500) were obtained from Sigma, and stock solutions in absolute ethanol were stored at −20 °C.

Damacena de Angelis C., Endoni B.T., Nuno D., Lamping K., Ledolter J., Koval O.M, & Grumbach I.M. (2022). Sex‐Specific Differences in Endothelial Function Are Driven by Divergent Mitochondrial Ca2+ Handling. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(13), e023912.

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Isolation and Western Blot Analysis

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Whole cell and tumour lysates were isolated using RIPA buffer supplemented with protease inhibitors (Complete Mini, Sigma-Aldrich Inc.) and phosphatase inhibitors including sodium pyrophosphate, β-glycerophosphate, sodium fluoride and sodium orthovanadate (Sigma-Aldrich). The following primary antibodies were used: anti-MCU (#D2Z3B 1:1000, Cell Signalling), anti-MICU1 (#HPA037479 1:1000, Sigma-Aldrich), anti-MICU2 (#ab101465 1:1000, Abcam), anti-phospho-SMAD3 (#C25A9, 1:1000, Cell Signalling), anti-SMAD3 (#9513, 1:1000, Cell Signalling), anti-MYOG (#sc-12732, 1:500, Santa-Cruz), anti-MHC preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted November 10, 2021. ; https://doi.org/10.1101/2021.11.10.468020 doi: bioRxiv preprint (#sc-32732, 1:250, Santa-Cruz) anti-HSP60 (#611563, BD Biosciences) and anti-β-actin (#A2228, 1:10,000, Sigma-Aldrich). Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.

Chiu H.Y., Loh A.H.P., & Taneja R. (2021). Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma.

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Mitochondrial Protein Localization Analysis

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Constructs, Cell Lines, siRNAs, and Antibodies Refer to Tables S4 andS5, respectively. Antibodies: anti-HA (Sigma and Roche), anti-FLAG (Sigma), anti-Strep (iba-lifesciences), anti-MICU1 (Sigma), anti-MICU2 (Thermo Scientific), anti-Smac (Sigma), anti-Mitofilin (Proteintech), anti-LDH (Santa Cruz Biotechnology), and anti-Mia40 (self-made; Fischer et al., 2013) . Secondary antibodies were directed against mouse or rabbit (BioRad). The following siRNAs were used: Hs_CHCHD4_5, Hs_CHCHD4_6, Hs_CBARA1_8, Hs_ CBARA1_12, Hs_EFHA1_7, and control siRNA (Quiagen).

Petrungaro C., Zimmermann K.M., Küttner V., Fischer M., Dengjel J., Bogeski I, & Riemer J. (2015). The Ca(2+)-Dependent Release of the Mia40-Induced MICU1-MICU2 Dimer from MCU Regulates Mitochondrial Ca(2+) Uptake. Cell metabolism, 22(4).

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