Colour measurements were made with a hand-held tristimulus reflectance spectrocolorimeter (Minolta Co., Model CM-508-d, Japan). For total anthocyanin and phenolic compound contents, and antioxidant capacity (TEAC assay), a UV- VIS spectrophotometer (model V-630, JASCO, Japan) was used. For the determination of antioxidant capacity evaluated by ORAC assay, fluorescence measurements were made by means of a Multilabel Microplate Reader (Perkin Elmer, Victor3 model, USA). Fluorescence measurements in Caenorhabditis elegans assays were performed in a Spark 20M Multimode Microplate Reader (Tecan, NC, USA).
AAPH (2,2′-Azobis (2-methylpropionamidine) dihydrochloride, ABTS (2,2 -azinobis (3-ethylbenzothiazoline)-6-sulfonate), fluorescein sodium salt, gallic acid (GA), Trolox (C14H18O4) and 2,7 – dichlorodihydrofluorescein diacetate (H2DCF-DA) were purchased from Sigma-Aldrich (St. Louis, USA). Ethyl alcohol, sodium acetate, sodium carbonate, and Tween 80 were from Biopack (Buenos Aires, Argentina). Folin Ciocalteau reagent, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, hydrochloric acid, potassium chloride, sodium chloride, sodium hydroxide, manganese sulphate, and malt extract agar (MEA) were purchased from Merck (Darmstadt, Germany).
AAPH (2,2′-Azobis (2-methylpropionamidine) dihydrochloride, ABTS (2,2 -azinobis (3-ethylbenzothiazoline)-6-sulfonate), fluorescein sodium salt, gallic acid (GA), Trolox (C14H18O4) and 2,7 – dichlorodihydrofluorescein diacetate (H2DCF-DA) were purchased from Sigma-Aldrich (St. Louis, USA). Ethyl alcohol, sodium acetate, sodium carbonate, and Tween 80 were from Biopack (Buenos Aires, Argentina). Folin Ciocalteau reagent, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, hydrochloric acid, potassium chloride, sodium chloride, sodium hydroxide, manganese sulphate, and malt extract agar (MEA) were purchased from Merck (Darmstadt, Germany).