Secondary antibodies conjugated to alexa 488

1-methyl4-phenyl-1,2,3,5-tetrahydropyridine (MPTP), 1-methyl-4-phenyl pyridium (MPP⁺), paraquat (PQ), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Anti-tyrosine hydroxylase (TH) antibody (#ab112) was obtained from Abcam (Cambridge, United Kingdom). Antibodies against phospho-Ser473-Akt (#9271S), total Akt (#9272S), phospho-Ser9-GSK3β (#9336S), total GSK3β (#9315S) were purchased from Cell Signaling Technology (Danvers, MA, United States). Anti-GAPDH (#60004-1-Ig) was purchased from ProteinTech Group (Rosemont, IL, United States). Secondary antibodies conjugated to Alexa 488 were purchased from Invitrogen (Carlsbad, CA, United States).

An MTT assay kit was purchased from Roche. A site-directed mutagenesis kit was purchased from Stratagene. 6-Hydroxydopamine hydrobromide (6-OHDA), DMSO, SB216763 (S3442, an inhibitor of GSK3β) and AGK2 (A8231, an inhibitor of SIRT2) were obtained from Sigma-Aldrich. Antibodies against pGSK3β (Ser9) and GSK3β were purchased from Cell Signaling (Danvers, MA, USA). Antibodies against SIRT2, ace-tubulin, α-tubulin, HA and Flag were purchased from Sigma-Aldrich. Secondary antibodies conjugated to Alexa 488 or Alexa 594 were purchased from Invitrogen. Hoechst 33258 (94403) was purchased from Sigma-Aldrich. Protein A/G-coated Sepharose beads were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). An anti-phosphoserine/threonine/tyrosine antibody was obtained from Abcam (ab15556). Protein kinase CDK5/p25 (cat. 14—516) and GSK3β (cat. 14—306) were purchased from Millipore. Cells were transfected using Lipofectamine 2000 Transfection Reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Other chemicals and reagents were of the highest analytical grade and were purchased from local commercial sources.