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Cd68 biotinylated plus streptavidin conjugated with qdot605

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CD68 (-biotinylated plus streptavidin conjugated with Qdot605) is a lab equipment product. It is a conjugate of biotinylated CD68 antibody and streptavidin labeled with Qdot605 fluorescent dye.

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Lab products found in correlation

Foxp3 pe, by Thermo Fisher Scientific (2 mentions) Intracellular fixation and permeabilization buffer set, by Thermo Fisher Scientific (2 mentions) Cd3 efluor450, by Thermo Fisher Scientific (2 mentions) Mabs for flow cytometry specific, by Thermo Fisher Scientific (2 mentions) Nos2 pe, by Thermo Fisher Scientific (2 mentions) Ifn γ apc, by Thermo Fisher Scientific (2 mentions) Tnf α pe, by Thermo Fisher Scientific (2 mentions) Rorγt pe, by Thermo Fisher Scientific (2 mentions) Gata 3 pe, by Thermo Fisher Scientific (2 mentions) F4 80, by Thermo Fisher Scientific (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (2 mentions) Cd11b, by Thermo Fisher Scientific (1 mentions) Cd80 pe, by Thermo Fisher Scientific (1 mentions) Dc sign, by Thermo Fisher Scientific (1 mentions) Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Ly 6c efluor 450, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd68 biotinylated plus streptavidin conjugated with qdot605

1

Flow Cytometric Analysis of Lung Immune Cells

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Isolated leukocytes from lungs of uninfected control as well as infected mice were stained with labeled monoclonal antibodies (mAbs) specific for cell surface markers or intracellular transcription factors or cytokines. mAbs for flow cytometry specific for CD11c (-Alexa-fluor 700), CD11b (-PECy7 or -APCCy7), MHCII (-PE), F4/80 (-PerCP-Cy5.5), DCsign (-APC), CD8(-APCCy7 -PECy5), CD4 (-FITC), Ly6c (-efluor-450), CD80 (-PE), CD86 (-PECy5), CD115 (-FITC), CD3 (-FITC), and CD3 (-efluor-450) were obtained from eBioscience and CD68 (-biotinylated plus streptavidin conjugated with Qdot605) was obtained from Invitrogen. The intracellular fixation and permeabilization buffer set of eBioscience was used to perform IFN-γ-APC, TNF-α-PE, and NOS2-PE. To Tbet-PE, GATA-3-PE, RORγT-PE, and Foxp3-PE transcription factors staining the Foxp3 staining buffer set was used (eBioscience).
Galvão-Filho B., de Castro J.T., Figueiredo M.M., Rosmaninho C.G., Antonelli L.R, & Gazzinelli R.T. (2019). The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4. Mucosal Immunology, 12(2), 312-322.
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2

Multiparameter Flow Cytometry of Mouse Lung Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated leukocytes from lungs of uninfected control as well as infected mice were stained with labeled monoclonal antibodies (mAbs) specific for cell surface markers or intracellular transcription factors or cytokines. mAbs for flow cytometry specific for CD11c (-Alexa-fluor 700), CD11b (-PECy7 or -APCCy7), MHCII (-PE), F4/80 (-PerCP-Cy5.5), DCsign (-APC), CD8(-APCCy7 -PECy5), CD4 (- FITC), Ly6c (-efluor-450), CD80 (-PE), CD86 (-PECy5), CD115 (-FITC), CD3 (-FITC) and CD3 (-efluor-450) were obtained from eBioscience and CD68 (-biotinylated plus streptavidin conjugated with Qdot605) was obteined from Invitrogen. The intracellular fixation and permeabilization buffer set of eBioscience was used to perform IFN-γ- APC, TNF-α-PE and NOS2-PE. To Tbet-PE, GATA-3-PE, RORγT-PE and Foxp3-PE transcription factors staining the Foxp3 staining buffer set was used (eBioscience).
Galvão-Filho B., de Castro J.T., Figueiredo M.M., Rosmaninho C.G., Antonelli L.R, & Gazzinelli R.T. (2019). The emergence of pathogenic TNF/iNOS producing dendritic cells (Tip-DCs) in a malaria model of acute respiratory distress syndrome (ARDS) is dependent on CCR4. Mucosal Immunology, 12(2), 312-322.
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