4 3 4 iodophenyl 2 4nitrophenyl 2h 5 tetrazolium 1 3 benzenedisulfonate wst 1 assay

HPLC-grade acetonitrile and methanol were purchased from Labscan (Dublin, Ireland). Acetic acidof analytical grade (assay >99.5%) was purchased from Fluka (Buchs, Switzerland). Water was purified by using a Milli-Q System (Millipore, Bedford, MA, USA). Other reagents unmarked were of analytical grade. methanol (MeOH) was purchased from Carlo Erba (Rodano, MI, Italy), H₂O₂ was bought from Farmac-Zabban S.p.a. (Calderara di Reno, BO, Italy), NaCO₃, gelatin and Hoechst 33342 were obtained from Sigma-Aldrich (Milan, Italy), poly(vinylidenefluoride-co-trifluoroethylene) [P(VDF-TrFE)] powder (70:30 % mol) was purchased from Piezotech Arkema (Pierre-Benite, France), Methyl ethyl ketone (MEK) was bought from Merck (Darmstadt, Germany). Medium 199 (M199), fetal bovine serum (FBS), penicillin-streptomycin solution, L-glutamine, HEPES buffer were supplied by Hospira S.r.l. (Naples, Italy).
4-[3-(4-iodophenyl)-2-(4nitrophenyl)-2H-5-tetrazolium]-1,3-benzenedisulfonate (WST-1 assay), was purchased from Roche Applied Science (Mannheim, Germany), 5-(and-6)-chloromethyl-2’,7’-dichloro-di-hydro-fluorescein diacetate, acetyl ester (CM-H₂DCFDA) was supplied by Invitrogen. EGM-2 medium bullet kit was bought from Lonza (Verviers, Belgium), alamarBlue^® was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Hexane, acetone, and Folin-Ciocalteau reagent, and gelatin were purchased from Sigma-Aldrich (Milan, Italy). HPLC grade formic acid and methanol were purchased from VWR (Milan, Italy). HPLC grade water (18 mΩ) was obtained by a Mill-Q purification system (Millipore Corp., Bedford, MA, USA).
H₂O₂ was purchased from Farmac-Zabban S.p.a. (Calderara di Reno, BO, Italy). Medium 199 (M199), fetal bovine serum (FBS), penicillin-streptomycin solution, L-glutamine, and HEPES buffer were supplied by Hospira S.r.l. (Naples, Italy).
4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolium]-1,3-benzenedisulfonate (WST-1 assay), was purchased from Roche Applied Science (Mannheim, Germany), 5-(and-6)-chloromethyl-2′, 7′-dichloro-di-hydro-fluorescein diacetate, and acetyl ester (CM − -H₂DCFDA) were supplied by Thermo Fisher Scientific Inc. (Waltham, MA, USA).

Hexane, acetone and Folin-Ciocalteau reagent were purchased from Sigma-Aldrich (Milan, Italy). H₂O₂ was bought from Farmac-Zabban S.p.a. (Calderara di Reno, BO, Italy); gelatin was obtained from Sigma-Aldrich (Milan, Italy). Medium 199 (M199), fetal bovine serum (FBS), penicillin-streptomycin solution, l-glutamine and HEPES buffer were supplied by Hospira S.r.l. (Naples, Italy). 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolium]-1,3-benzenedisulfonate (WST-1 assay) was purchased from Roche Applied Science (Mannheim, Germany); 5-(and-6)-chloromethyl-2′,7′-dichloro-di-hydro-fluorescein diacetate, acetyl ester (CM-H₂DCFDA) was supplied by Invitrogen (Thermo Fisher Scientific, Vantaa, Finland).