FT-IR spectra was obtained by using an FT-IR spectrophotometer (Bruker Alpha Instrument). Using FT-IR spectra, functional groups present can be detected. It was performed to detect if any crosslinking has taken place in the collagen nanoparticle after adding EDC-Hcl and MDA. FT-IR spectrum was recorded in the frequency range of 4,000 to 400 cm–1. Particle size (PS) and polydispersity index (PDI) were determined using dynamic light scattering (DLS) technique on a Zetasizer Nano ZS (Malvern Instruments Corp., Malvern, United Kingdom). All samples were diluted with Millipore-filtered deionized water to an appropriate scattering intensity. The nanoformulation was morphologically examined using a technique called transmission electron microscopy (TEM) (TALOS Instrument, Thermo Fischer Scientific, United States) at AIIMS, New Delhi. The sample preparation involved putting a few drops of nanoparticles on a carbon-coated copper grid and was negatively stained using 1% phosphotungstic acid (PTA). Excess of stain solution was removed using a filter paper followed by air drying. The next step required the examination of stained films using a TEM instrument.
Talos instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TALOS Instrument is a high-performance analytical tool designed for materials characterization. It provides advanced electron microscopy capabilities, enabling detailed analysis of a wide range of samples. The core function of the TALOS Instrument is to capture high-resolution images and collect valuable data about the structure and composition of materials at the nanoscale level.
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Characterization of Collagen Nanoparticles
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Characterization of Collagen Nanoparticles
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Fourier transform infrared spectroscopy (FT-IR) was done to analyze if crosslinking has taken place in collagen nanoparticle on the addition of EDC·HCl and MDA. It was done on a Bruker alpha instrument and the spectrum was recorded in the range 4000 cm−1 to 400 cm−1. Dynamic Light Scattering (DLS) analysis was done to determine nanoparticle size distribution and its average size determined using Zetasizer Nano ZS (Malvern Instruments Corp., Malvern, UK). Size measurement was done at 4 °C using a disposable folded capillary cell. Transmission Electron Microscopy (TEM) analysis gives nanoparticle shape and size, few drops of nanoparticles were dropped cast on a copper grid and negative staining done using 1% PTA. TEM was done at AIIMS, New Delhi on a TALOS instrument (Thermo Fischer Scientific, USA).
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