This study used the following mAbs and reagents: Allophycocyanin (APC)-Cy7-conjugated anti-CD3, APC-conjugated anti-CD3, APC-conjugated anti-CD69, and fluorescein isothiocyanate (FITC)-conjugated anti-CD45, FITC-conjugated anti-CD56, FITC-conjugated anti-CD107a, FITC-conjugated anti-IFN-γ, phycoerythrin (PE)-conjugated anti-CD3, PE-conjugated anti-CD56, PE-conjugated anti-CD69, PE-Cy7-conjugated anti-TNF-α, PerCP-conjugated anti-CD3, PerCP-conjugated anti-CD45, FITC-conjugated mouse IgG isotype and PE-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA). Cells were stained with combinations of appropriate mAb for 20 minutes at 4°C. Stained cells were analyzed on a Navios flow cytometer using Kaluza software (version 1.1; Beckman Coulter, Brea, CA).
Pe conjugated mouse igg isotype control
Manufactured by BD
Sourced in United States
The PE-conjugated mouse IgG isotype control is a laboratory reagent used as a reference control in flow cytometry experiments. It is a mouse immunoglobulin G (IgG) antibody conjugated with the fluorescent dye Phycoerythrin (PE). This control is used to establish background fluorescence levels and to set appropriate gates and thresholds during the analysis of cell samples.
Automatically generated - may contain errors
Lab products found in correlation
Navios flow cytometer, by Beckman Coulter (2 mentions)
Apc cy7 conjugated anti cd3, by BD (1 mentions)
Fitc conjugated anti cd107a, by BD (1 mentions)
Fitc conjugated anti ifn γ, by BD (1 mentions)
Pe conjugated anti cd3, by BD (1 mentions)
Percp conjugated anti cd45, by BD (1 mentions)
Pe conjugated anti cd56, by BD (1 mentions)
Pe conjugated anti cd69, by BD (1 mentions)
Pe cy7 conjugated anti tnf α, by BD (1 mentions)
Percp conjugated anti cd3, by BD (1 mentions)
Fitc conjugated mouse igg isotype, by BD (1 mentions)
Cytotoxicity ldh assay kit, by Dojindo Laboratories (1 mentions)
Cellquest pro software, by BD (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Acutase, by Innovative Cell Technologies (1 mentions)
Facscalibur system, by BD (1 mentions)
4 protocols using pe conjugated mouse igg isotype control
1
Multiparameter Flow Cytometry Immunophenotyping
2
Multiparameter Flow Cytometry Analysis of Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following monoclonal antibodies (mAbs) and reagents were used in this study: fluorescein isothiocyanate (FITC)-conjugated Lineage Cocktail 1(CD3, CD14, CD16, CD19, CD20, CD56), phycoerythrin (PE)-conjugated anti-CD123, anti-CD86, and anti-CD274; allophycocyanin (APC)-conjugated anti-CD11c, anti-CD86, and anti-CD274; BV421-conjugated anti-HLA-DR; Alexa Fluor 647-conjugated anti-IFN-α; PE-conjugated anti-interleukin-12 (anti-IL-12), PE-Cy7-conjugated anti-tumor necrosis factor-α (anti-TNF-α) mAb, and PE-conjugated mouse IgG isotype control (all from Becton Dickinson, San Diego, CA, USA). The cells were stained with combinations of the appropriate mAbs for 20 minutes at 4°C. The stained cells were analyzed on a Navios flow cytometer using Kaluza software (version 1.5a; Beckman Coulter, Brea, CA, USA).
3
Cytotoxicity of NK-92 cells against cancer cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
αFR expression on the surface of cancer cells was investigated by flow cytometry using PE-conjugated mouse anti-human FOLR1 antibody (R&D SYSTEMS, Minneapolis) and PE-conjugated mouse IgG isotype control (BD Biosciences). Flow cytometry was performed using a BD FACS Canto II flow cytometer (BD Biosciences), and the data were analyzed using CellQuest Pro software (BD Biosciences). The cytotoxicity of the effector cells (NK-92 cells and transfected NK-92 cells) was measured using the LDH cytotoxicity assay kit (DOJINDO, Tokyo, Japan). After coculture of the target cells and the effector cells for 18 hours in 96-well plates at different effector-to-target (E/T) ratios, the supernatants were collected. According to the manufacturer’s instructions, the LDH content in the supernatants was detected, and the specific cell lysis of the effector cells at different E/T ratios was calculated.
4
Cell Surface Marker Profiling by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were treated with Acutase (Innovative Cell Technologies, San Diego, CA, USA) at 37 °C for 10 min. Dissociated cells were rinsed twice with FACS buffer (PBS containing 5% fetal bovine serum) by centrifugation at 300× g for 5 min. The cells were labeled with fluorophore-conjugated antibodies on ice for 30 min. Finally, the cells were rinsed twice with FACS buffer by centrifugation at 300× g for 5 min, and assayed using a FACSCalibur system (Becton Dickson, San Jose, CA, USA) according to the manufacturer’s instructions. Data were analyzed using the FlowJo software, version 7.2.5 (Tree Star, Inc., Ashland, OR, USA). Phycobiliproteins (PE)-conjugated mouse IgG isotype control (1:100, BD Biosciences, Bedford, MA, USA), PE-mouse anti human SSEA4 (1:100, BD Biosciences, Bedford, MA, USA), allophycocyanin (APC)-conjugated mouse IgG isotype control (1:100, BD Biosciences, Bedford, MA, USA), and APC-mouse anti human CXCR4 antibody (1:100, BD Biosciences, Bedford, MA, USA) were used in this study.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!