For serum samples, 50 μL serum was added to 100 μL cold acetonitrile solution containing 1 μM d5-TCA (Toronto Research Chemicals) as an internal standard. For tissue samples, 20 mg of tissue were homogenized with an acetonitrile solution containing 1 μM d5-TCA. After being precipitated, the upper supernatants were mixed with 0.1% formic acid solution. Then the concentrations of BAs were determined by Acquity UPLC/Synapt XS HDMS system (Waters Corp.). Chromatographic separation was achieved on an Acquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm, Waters Corp.) at 45 °C with a gradient elution of mobile phase of 0.1% formic acid in water and 0.1% formic acid in acetonitrile (v/v) at a flow rate of 0.4 mL/min. Mass spectrometry was carried out under electrospray negative (ESI-) ion mode. A mass range of m/z 50 to 850 was acquired.
D5 tca
Manufactured by LGC
Sourced in Canada
The D5-TCA is a laboratory equipment product designed for performing analytical tests and procedures. It serves as a core component in various laboratory applications, providing essential functionalities for researchers and analysts. The detailed specifications and intended use of this product are not available within the scope of this response.
Automatically generated - may contain errors
Lab products found in correlation
Acquity beh c18 column, by Waters Corporation (1 mentions)
Deoxycholic acid, by Merck Group (1 mentions)
Taurohyodeoxycholic acid, by Merck Group (1 mentions)
Taurodeoxycholic acid, by Merck Group (1 mentions)
Tauroursodeoxycholic acid tudca, by Merck Group (1 mentions)
β mca, by LGC (1 mentions)
Tauro chenodeoxycholic acid t cdca, by Merck Group (1 mentions)
Acquity hss t3 column, by Waters Corporation (1 mentions)
3 protocols using d5 tca
1
Quantification of Bile Acids in Serum and Tissue
2
Bile Acid Supplementation Protocol
3
Fecal Bile Acid Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Feces were prepared as previously described (Chen et al., 2016) . In brief, 40 mg of feces was mixed with 400 μL of 80% acetonitrile; then, 10 μg/mL D5-TCA (Toronto Research Chemicals Inc., Toronto, ON, Canada) or D4-CA (CDN Isotopes Inc., Pointe-Claire, QC, Canada) was added as internal standard. The final concentrations of D5-TCA and D4-CA were both 0.2 μg/mL. The supernatant was collected and subjected to LC/MS analysis. Bile acids were analyzed by an AB SCIEX QTRAP 4500 LC/MS/MS system with an Acquity HSS T3 column (Waters, Milford, MA), and gradient elution with 10 mM formic acid in water and 10 mM formic acid in acetonitrile: methanol (35:65) as mobile phases. The cone voltage and collision energy were set as 70 V and 2 eV for nonconjugated bile acid, and 90 V and 65 eV for taurine conjugates, respectively. All standard solutions were stored at -20°C. Supplemental Table S2 (https:// doi .org/ 10 .3168/ jds .2017 -13713) shows the retention times of the analytes.
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