Opn sc 21742

Manufactured by Santa Cruz Biotechnology

Sourced in United States

OPN (sc-21742) is a primary antibody product offered by Santa Cruz Biotechnology. It is a laboratory reagent used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Pcr primer, by Bioneer (1 mentions) Lipofectamine 2000 sirna transfection reagent, by Thermo Fisher Scientific (1 mentions) Platelet derived growth factor, by Merck Group (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Hek293 cell, by American Type Culture Collection (1 mentions) Cox 4, by Cell Signaling Technology (1 mentions) C3h10t1 2, by American Type Culture Collection (1 mentions) Dylight 594 conjugated secondary antibody a23410, by Abbkine (1 mentions) Alizarin red s st1078, by Beyotime (1 mentions) Gapdh 10494 1 ap, by Proteintech (1 mentions) Goat anti mouse igg 115 035 003, by Jackson ImmunoResearch (1 mentions) Gapdh 2118, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg 111 035 003, by Jackson ImmunoResearch (1 mentions) Novex ecl chemiluminescent substrate reagent kit, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions)

Close spelling variants of this product

Sc-21742 (11 citations)

4 protocols using opn sc 21742

PDGF Signaling Modulation via AP-1 and C/EBPβ

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Platelet derived growth factor (PDGF) was purchased from Sigma (St. Louis, MO), and OPN (sc-21742) and β-actin (sc-47778) antibodies were purchased from Santa Cruz Biotechnology Inc. (Beverly, MA). Horseradish peroxidase (HRP)-conjugated IgG antibody (Santa Cruz Biotechnology Inc.) was used as the secondary antibody. PCR primers were from Bioneer (Seoul). AP-1 (10024-2-AP) antibody was purchased from Proteintech (Proteintech Group, Chicago, USA), and C/EBPβ (ab15049) antibody from Abcam (Cambrige, MA). Restriction enzymes were supplied by Promega (Madison, WI).
AP-1 and C/EBPβ siRNA oligonucleotides were synthesized by Bioneer (Daejeon, Korea). siRNA molecules were transfected into cells using Lipofectamine 2000 siRNA transfection reagent (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions. siRNA sequences against AP-1 and C/EBPβ were as follows: AP-1, ACUGUAGAUUGCUUCUGUA (sence) and UACAGAAGCAAUCUACAGU (antisense); C/EBPβ, GACAAGCUGAGCGACGAGU (sence) and ACUCGUCGCUCAGCUUGUC (antisense).

Jang M.A., Lee S.J., Baek S.E., Park S.Y., Choi Y.W, & Kim C.D. (2017). α-Iso-Cubebene Inhibits PDGF-Induced Vascular Smooth Muscle Cell Proliferation by Suppressing Osteopontin Expression. PLoS ONE, 12(1), e0170699.

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Cell Culture and Osteogenic Differentiation

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C3H10T1/2, C2C12, and HEK-293 cell lines were purchased from ATCC (Manassas, VA, USA). Cells were cultured in dishes, flasks, or plates with Dulbecco's modified Eagle's medium which contained 10% fetal bovine serum (FBS), streptomycin (100 μg/mL), and penicillin (100 U/mL). These dishes, flasks, and plates were cultured in 37°C incubators with 5% CO₂. Primary antibodies against Runx2 (sc-390715), OPN (sc-21742), β-catenin (sc-7963), GSK-3β (sc-81462), p-GSK-3β (sc-81496), and PDK4 (sc-518103) were bought from Santa Cruz Biotechnology (CA, USA); GAPDH (10494-1-AP) was purchased from Proteintech (Wuhan, China); COXIV was ordered from Cell Signaling Technology (Boston, USA). Dylight 594 conjugated secondary antibody (A23410) was bought from Abbkine (Chinese branch). Alizarin Red S (ST1078) and Alkaline Phosphatase Assay Kit (C3206) were obtained from Beyotime (Shanghai, China).

Yang Y.Y., Deng Y.X., Yao X.T., Luo H.H., He W.G., Cao X.L., Chen R.C., He B.C., Jiang H.T, & Wang J. (2023). lncRNA MEG3 Promotes PDK4/GSK-3β/β-Catenin Axis in MEFs by Targeting miR-532-5p. Oxidative Medicine and Cellular Longevity, 2023, 3563663.

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Immunoblot Analysis of Osteoprogenitor Cells

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Stimulated osteoprogenitor cells were lysed with 1X RIPA buffer containing phosphatase (5870S, Cell Signaling Technology, USA) and protease (535140, Calbiochem, USA) inhibitors. Proteins were quantified using the Bradford assay. Total cell protein (10–30 μg) was subjected to immunoblotting using the following antibodies: β-catenin (9562S), active β-catenin (19807S), phos-AKT (Ser473) (4060), total-AKT (4691), phos-ERK (9101), total-ERK (9102), and GAPDH (2118) from Cell Signaling Technology (Danvers, MA, USA). CDKN1A (p21; A19094) and OCN (ab13420) antibodies were obtained from Abaclonal and abcam, respectively. WNT16 (sc-271897), CASP3 (sc-7272), and OPN (sc-21742) antibodies were obtained from Santa Cruz Biotechnology. Goat anti-rabbit IgG (111-035-003) and goat anti-mouse IgG (115-035-003) secondary antibodies were obtained from Jackson ImmunoResearch (West Grove, PA, USA).

Jo S., Weon S., Nam B., Jang M.A., Kang H., Kim T.J., Park Y.S, & Kim T.H. (2021). WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis. Arthritis Research & Therapy, 23, 301.

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Western Blot Analysis of Osteopontin

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Total proteins were extracted from the gastrocnemius muscle and separated by 13% SDS-PAGE polyacrylamide gel electrophoresis and were transferred onto a nitrocellulose membrane (GE Healthcare Biosciences, Pittsburgh, PA, USA) for 60 min at 0.35A at 4°C. Membranes were then pre-stained in 0.2% Ponceau S, to ensure protein transfer and equal protein loading of the lanes. Membranes were blocked with 5% non fat milk in PBS, 0.1% Tween 20 (TBS-T) for 1 h and probed using the primary antibodies against OPN sc-21742 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). After an overnight period of incubation, membranes were washed three times with TBS-T for 10 min. The blots were then immunostained with Pierce^® Anti-mouse IgG (HRP) Polyclonal antibody (Thermo Fisher Scientific, Waltham, MA, USA) and posterior detection of protein was done using Novex^® ECL Chemiluminescent Substrate Reagent Kit (Invitrogen, Waltham, MA, USA).
Quantitative analysis of mouse OPN present in total protein extracted was performed using the ImageJ software (http://rsb.info.nih.gov/ij/), with myosin at the Ponceau staining as a protein loading control. The value of each animal was normalized to the normal control within the same blot.

Calyjur P.C., Almeida C.D., Ayub-Guerrieri D., Ribeiro AF J.u.n.i.o.r., Fernandes S.D., Ishiba R., dos Santos A.L., Onofre-Oliveira P, & Vainzof M. (2016). The mdx Mutation in the 129/Sv Background Results in a Milder Phenotype: Transcriptome Comparative Analysis Searching for the Protective Factors. PLoS ONE, 11(3), e0150748.

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