Bortezomib was purchased from ChemieTek (Indianapolis, IN). Thapsigargin, cycloheximide, and propidium iodide (PI) and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies were purchased from the following sources: phospho-eIF2α (#3398S), GCN2 (#3302), PERK (#3192) and lamin A/C (#4777) (Cell Signaling Technology, Beverly, MA), eIF2α, (#44-728G, Invitrogen/Life Technologies, Grand Island, NY), HRI (#sc-30143), ubiquitin (#sc-8017) and ATF4 (#sc-200) (Santa Cruz Biotechnology, Santa Cruz, CA), phospho-GCN2 (#ab75836, Abcam, Cambridge, MA), β-actin (#A5316, Sigma-Aldrich), and anti-mouse/anti-rabbit HRP-labeled secondary antibodies (#W4021/#W4011, Promega, Madison, WI).
Phospho gcn2
Manufactured by Abcam
Phospho-GCN2 is a lab equipment product that detects the phosphorylated form of the GCN2 protein. GCN2 is a serine/threonine protein kinase that plays a role in the cellular response to amino acid starvation. The Phospho-GCN2 product can be used to measure the activation state of GCN2 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ubiquitin, by Santa Cruz Biotechnology (2 mentions)
Eif2α, by Thermo Fisher Scientific (2 mentions)
Phospho eif2α, by Cell Signaling Technology (2 mentions)
Bortezomib, by Chemietek (2 mentions)
Anti mouse anti rabbit hrp labeled secondary antibodies, by Promega (2 mentions)
Lamin a c, by Cell Signaling Technology (2 mentions)
P erk, by Cell Signaling Technology (2 mentions)
Propidium iodide, by Merck Group (2 mentions)
Cycloheximide (chx), by Merck Group (2 mentions)
β actin, by Merck Group (2 mentions)
Thapsigargin, by Merck Group (2 mentions)
N acetyl l cysteine, by Merck Group (2 mentions)
2 protocols using phospho gcn2
1
Cellular Stress Response Signaling
2
Cellular Stress Response Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bortezomib was purchased from ChemieTek (Indianapolis, IN). Thapsigargin, cycloheximide, and propidium iodide (PI) and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies were purchased from the following sources: phospho-eIF2α (#3398S), GCN2 (#3302), PERK (#3192) and lamin A/C (#4777) (Cell Signaling Technology, Beverly, MA), eIF2α, (#44-728G, Invitrogen/Life Technologies, Grand Island, NY), HRI (#sc-30143), ubiquitin (#sc-8017) and ATF4 (#sc-200) (Santa Cruz Biotechnology, Santa Cruz, CA), phospho-GCN2 (#ab75836, Abcam, Cambridge, MA), β-actin (#A5316, Sigma-Aldrich), and anti-mouse/anti-rabbit HRP-labeled secondary antibodies (#W4021/#W4011, Promega, Madison, WI).
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