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Fitc conjugated anti α smooth muscle actin α sma monoclonal antibody

Manufactured by Merck Group
Sourced in United States

The FITC-conjugated anti-α-smooth muscle actin (α-SMA) monoclonal antibody is a laboratory reagent used for the detection and localization of α-SMA, a protein found in smooth muscle cells. The antibody is conjugated with the fluorescent dye FITC, allowing for visualization and analysis of α-SMA expression in various biological samples.

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3 protocols using fitc conjugated anti α smooth muscle actin α sma monoclonal antibody

1

Investigating Cellular Signaling in Cells

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LIRA was purchased from Novo Nordisk (Bagsvaerd, Denmark). The ERK1/2 inhibitor (PD98059), PI3K inhibitor (LY294002), and FITC-conjugated anti-α-smooth muscle actin (α-SMA) monoclonal antibody were obtained from Sigma-Aldrich (St Louis, MO, USA). The GLP-1R antagonist, Exendin (9–39) (Exe(9–39)), was purchased from AnaSpec (San Jose, CA, USA). Trypsin, fetal bovine serum (FBS), and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (of Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal antibodies against ERK1/2, Akt and phosphorylated (p)-Akt, and the mouse monoclonal antibody against p-ERK1/2 were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit polyclonal antibodies against β-actin, Bax, Bcl-2, and caspase-3 were purchased from Abcam (Cambridge, UK). Secondary antibodies were purchased from Vector Labs (Burlingame, CA, USA). Western blot bands were detected using the ECL Advance Western blotting detection kit from GE Healthcare (Chalfont St Giles, UK). Cell Counting Kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, USA). Transwell plates were purchased from Millipore (Bedford, MA, USA). Annexin V-FITC Kit was purchased from BD Biosciences (San Jose, CA, USA).
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2

Immunohistochemical Evaluation of Infarct Tissue

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For evaluation of inflammation, a mouse anti-rat CD68 (Millipore, Temecula CA) was used followed by an Alexa fluor 594 goat anti-mouse antibody (Invitrogen, Carlsbad, CA). For evaluation of angiogenesis, ECs were detected by a rabbit anti-rat Von Willebrand factor (vWF) antibody (US Abcam, Cambridge, MA) followed by an Alexa fluor 594 goat anti-rabbit antibody (Invitrogen Carlsbad, CA). Mural cells were detected by a FITC-conjugated anti-α- smooth muscle actin (α-SMA) monoclonal antibody (Sigma Aldrich, St. Louis, MO). Viable cardiomyocytes were detected by staining using a mouse anti-rat cardiac troponin I (cTnI) antibody (US Abcam, Cambridge, MA) followed by an Alexa fluor 488 goat anti-mouse antibody (Invitrogen, Carlsbad, CA). All slides were last counterstained with DAPI (Invitrogen, Carlsbad, CA).
For quantification, four to five slides from each group were utilized near the midsection level of the infarct tissue. The numbers of CD68-positive cells and vWF- and α-SMA-positive vessels were counted and reported per mm2 areas. The cTnI-positive fraction area in the infarct region was measured by NIS Elements AR software. Intensity of fluorescence was determined by ImageJ and normalized to the background value.
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3

Sitagliptin Regulates Osteoblast Differentiation

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Sitagliptin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The ERK1/2 inhibitor (PD98059) and FITC-conjugated anti-α-smooth muscle actin (α-SMA) monoclonal antibody were obtained from Sigma-Aldrich (St Louis, MO, USA). Mouse monoclonal core-binding factor α 1 (cbfa1) (ab54868) and mouse monoclonal α-SMA antibody (ab7817) were obtained from Abcam (Cambridge, MA, USA). Trypsin, fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM) were purchased from Gibco (of Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal antibody against ERK1/2 and a mouse monoclonal antibody against p-ERK1/2 were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Secondary antibodies were purchased from Vector Labs (Burlingame, CA, USA). Western blot bands were detected using the ECL Advance Western blotting detection kit from GE Healthcare (Chalfont St Giles, UK). Cell counting kit-8 was purchased from Dojindo Molecular Technologies (Rockville, MD, USA). Transwell plates were purchased from Millipore (Bedford, MA, USA). Annexin V-FITC Kit was purchased from BD Biosciences (San Jose, CA, USA). The alkaline phosphatase (ALP) activity kit was from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Calcium Colorimetric Assay Kit was obtained from Biovision (Mountain View, CA, USA).
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